Asia Pac Allergy.  2016 Oct;6(4):236-244. 10.5415/apallergy.2016.6.4.236.

Imidacloprid inhibits IgE-mediated RBL-2H3 cell degranulation and passive cutaneous anaphylaxis

Affiliations
  • 1State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. chenhongbing@ncu.edu.cn
  • 2School of Food Science, Nanchang University, Nanchang 330047, China.
  • 3Department of Rehabilitation, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
  • 4Department of Anesthesiology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China.

Abstract

BACKGROUND
Imidacloprid has been commonly used as a pesticide for crop protection and acts as nicotinic acetylcholine receptor agonists. Little information about the relationship between imidacloprid and allergy is available.
OBJECTIVE
This study aims to examine the effects of imidacoprid on IgE-mediated mast cell activation.
METHODS
The rat basophilic leukemia cell line RBL-2H3 (RBL-2H3 cells) were treated with 10⁻³- 10⁻¹² mol/L imidacloprid, followed by measuring the mediator production, influx of Ca²âº in IgE-activated RBL-2H3 cells, and the possible effects of imidacoprid on anti-dinitrophenyl IgE-induced passive cutaneous anaphylaxis (PCA).
RESULTS
It was shown that imidacoprid suppressed the production of histamine, β-hexosaminidase, leukotriene C4, interleukin-6, tumor necrosis factor-α, and Ca²âº mobilization in IgE-activated RBL-2H3 cells and decreased vascular extravasation in IgE-induced PCA.
CONCLUSION
It is the first time to show that imidacloprid suppressed the activation of RBL-2H3 cells.

Keyword

Imidacloprid; Mast cells; Ca²⁺ influx

MeSH Terms

Animals
Basophils
Cell Degranulation*
Cell Line
Crop Protection
Histamine
Hypersensitivity
Interleukin-6
Leukemia
Leukotriene C4
Mast Cells
Necrosis
Passive Cutaneous Anaphylaxis*
Rats
Receptors, Nicotinic
Histamine
Interleukin-6
Leukotriene C4
Receptors, Nicotinic
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