Korean J Phys Anthropol.
2017 Jun;30(2):53-60. 10.11637/kjpa.2017.30.2.53.
Tryptophan Negatively Regulates IgE-mediated Mast Cell Activation
- Affiliations
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- 1Department of Medical Science, Chungnam National University, Korea. parksk@cnu.ac.kr
- 2Department of Oriental Medicine, Daejeon University, Korea.
- 3Department of Life Science, Daejeon University, Korea.
- 4Department of Radiation Oncology, Chungnam National University Hospital, Korea.
- 5Department of Biochemistry, College of Medicine, Chungnam National University, Korea.
- KMID: 2385297
- DOI: http://doi.org/10.11637/kjpa.2017.30.2.53
Abstract
- Mast cells are major immune cells in allergy to secrete allergic mediators by a degranulation process and make and secrete inflammatory lipids and cytokines in response to antigen stimulation. An amino acid tryptophan regulates immune functions. Tryptophan ameliorates inflammatory colitis in which mast cells are engaged. However, its effects on mast cells remain to be solved. We investigated the effect of tryptophan on IgE-mediated allergic responses in the mast cells and mice. IgE-mediated passive cutaneous anaphylaxis (PCA) in mice were examined. Also IgE-mediated mast cell activation responses such as degranulation of stored granules and secretion of inflammatory lipid LTB₄ and cytokines (TNF-α and IL-4) were measured. Intraperitoneal administration of tryptophan suppressed PCA in mice. Also, in the cellular level tryptophan inhibited IgE-mediated mast cell activation such as IgE-mediated degranulation and the production of LTB₄. Also, it inhibited production of inflammatory cytokines TNF-α and IL-4. In summary, tryptophan suppressed IgE-mediated allergic activation in vivo and in vitro. Tryptophan supplementation is beneficial for IgE-mediated allergy.
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