Korean J Pathol.  2009 Apr;43(2):107-112.

The Overexpression of Histone Deacetylase 1 and Its Relationship with p16INK4a Gene Hypermethylation in Pulmonary Squamous Cell Carcinoma and Adenocarcinoma

• Park JH
• Hong YS
• Choi PJ
• Kim NY
• Lee KE
• Roh MS

Abstract

BACKGROUND: DNA methylation and histone modification are dynamically linked in the epigenetic control of gene silencing and they play an important role in tumorigenesis.
METHODS
To evaluate the role of histone deacetylase 1 (HDAC1) in the development of lung cancer and the relationship between a HDAC1 overexpression and p16INK4a hypermethylation, we performed immunohistochemical staining for HDAC1 in 76 lung cancer specimens (39 squamous cell carcinomas and 37 adenocarcinomas) that had been previously evaluated for their p16INK4a methylation status by real-time quantitative polymerase chain reaction.
RESULTS
A HDAC1 overexpression (>50% of HDAC1 immunoreactive cells) was detected in 65 (85.5%) out of the 76 cases and it was more frequently seen in the squamous cell carcinomas (97.4%) than in the adenocarcinomas (73.0%) (p=0.002). The incidence of HDAC1 overexpression tended to be higher in the heavy smokers with more than 20 pack-years (p=0.067). Although there was no statistical significance, the frequency of p16INK4a hypermethylation in the cases with a HDAC1 overexpression (27.7%) tended to be higher than that in the cases without a HDAC1 overexpression (9.0%) (p=0.175).
CONCLUSIONS
A HDAC1 overexpression might be involved in lung carcinogenesis, and especially in a subgroup of smoking and squamous cell carcinoma patients, and a HDAC1 overexpression may be associated with p16INK4a hypermethylation.

Keyword

Lung; Squamous cell carcinoma; HDAC1 protein, human; Cyclin-dependent kinase inhibitor p16; Hypermethylation

MeSH Terms

Adenocarcinoma
Carcinoma, Squamous Cell
Cell Transformation, Neoplastic
Cyclin-Dependent Kinase Inhibitor p16
DNA Methylation
Epigenomics
Gene Silencing
Genes, p16
Histone Deacetylase 1
Histone Deacetylases
Histones
Humans
Incidence
Lung
Lung Neoplasms
Methylation
Polymerase Chain Reaction
Smoke
Smoking
Cyclin-Dependent Kinase Inhibitor p16
Histone Deacetylase 1
Histone Deacetylases
Histones
Smoke