J Korean Neurosurg Soc.  2010 Jan;47(1):26-29. 10.3340/jkns.2010.47.1.26.

Different Expression of Extracellular Matrix Genes: Primary vs. Recurrent Disc Herniation

• Kuh SU
• Kwon YM
• Chin DK
• Kim KS
• Jin BH
• Cho YE

• KMID: 1495085
• DOI: http://doi.org/10.3340/jkns.2010.47.1.26

Abstract

OBJECTIVE
Recurrent lumbar disc herniation has been reported to occur in 5% to 15% of surgically treated primary lumbar disc herniation cases. We investigated the molecular biologic characteristics of primary herniated discs and recurrent discs to see whether the recurrent discs has the similar biological features with primary herniated discs.
METHODS
Primary herniated disc and recurrent disc cells were obtained by discectomy of lumbar disc patients and cells were isolated and then taken through monolayer cultures. We compared chondrogenic and osteogenic mRNA gene expression, and western blot between the two groups.
RESULTS
The mRNA gene expression of recurrent disc cells were increased 1.47* times for aggrecan, 1.38 times for type I collagen, 2.04 times for type II collagen, 1.22 times for both Sox-9 and osteocalcin, and 1.31 times for alkaline phosphatase, respectively, compared with the primary herniated lumbar disc cells (*indicates p < 0.05). Western blot results for each aggrecan, type I collagen, type II collagen, Sox-9, osteocalcin, and alkaline phosphatase were similar between the primary herniated disc cells and recurrent disc cells.
CONCLUSION
These results indicate that the recurrent disc cells have similar chondrogenic and osteogenic gene expression compared to primary herniated disc cells. Therefore, we assumed that the regeneration of remaining discs could fill the previous discectomy space and also it could be one of the factors for disc recurrence especially in the molecular biologic field.

Keyword

Recurrent disc; Aggrecan; Collagen; Sox-9; Osteocalcin; Alkaline phosphatase

MeSH Terms

Aggrecans
Alkaline Phosphatase
Blotting, Western
Collagen
Collagen Type I
Collagen Type II
Diskectomy
Durapatite
Extracellular Matrix
Gene Expression
Humans
Intervertebral Disc Displacement
Osteocalcin
Population Characteristics
Recurrence
Regeneration
RNA, Messenger
Aggrecans
Alkaline Phosphatase
Collagen
Collagen Type I
Collagen Type II
Durapatite
Osteocalcin
RNA, Messenger