J Korean Neurosurg Soc.  2017 Jan;60(1):40-46. 10.3340/jkns.2015.0911.002.

Role of Expression of Inflammatory Mediators in Primary and Recurrent Lumbar Disc Herniation

• Dagistan Y
• Cukur S
• Dagistan E
• Gezici AR

• KMID: 2365585
• DOI: http://doi.org/10.3340/jkns.2015.0911.002

Abstract

OBJECTIVE
To assess role of some inflammatory mediators in patients with primary and recurrent lumbar disc herniation. Expression of IL-6, transforming growth factor (TGF)-1, insulin-like growth factor (IGF)-1, and Bcl-2-associated X protein (BAX) have been shown to be more intense in the primary group than the recurrent goup, but this mediators may be important aspects prognostic.
METHODS
19 patients underwent primary and revision operations between June 1, 2009 and June 1, 2014, and they were included in this study. The 19 patients' intervertebral disc specimens obtained from the primary procedures and reoperations were evaluated. Expression of IL-6, TGF-1, IGF-1, and BAX were examined immunohistochemically in the 38 biopsy tissues obtained from the primary and recurrent herniated intervertebral discs during the operation.
RESULTS
For IL-6 expression in the intervertebral disc specimens, there was no difference between the groups. The immunohistochemical study showed that the intervertebral disc specimens in the primary group were stained intensely by TGF-1 compared with the recurrent group. Expression of IGF-1 in the primary group was found moderate. In contrast, in the recurrent group of patients was mild expression of IGF-1. The primary group intervertebral disc specimens were stained moderately by BAX compared with the recurrent group.
CONCLUSION
The results of our prognostic evaluation of patients in the recurrent group who were operated due to disc herniation suggest that mediators may be important parameters.

Keyword

Inflammatory mediators; Disc herniation; Immunohistochemical

MeSH Terms

bcl-2-Associated X Protein
Biopsy
Humans
Insulin-Like Growth Factor I
Interleukin-6
Intervertebral Disc
Transforming Growth Factors
Insulin-Like Growth Factor I
Interleukin-6
Transforming Growth Factors
bcl-2-Associated X Protein

Figure

• 

  Fig. 1 Immunohistochemical staining of intervertebral disc for IL-6 expression. A : IL-6 expression in chondrocytes of the intervertebral disc was moderate in the primary group (Immun-Peroxidase, original magnification 100). B : In the recurrent group, in chondrocytes of the intervertebral disc were stained mild by IL-6 (Immuno-Peroxidase, original magnification 100).

• 

  Fig. 2 Immunohistochemical staining of the intervertebral disc for TGF. A : The chondrocytes of the intervertebral disc in primary group were stained intensely by TGF (Immun-Peroxidase, original magnification 100). B : In contrast, the chondrocytes of the intervertebral disc in the recurrent group were stained moderate by TGF (Immuno-Peroxidase, original magnification 100). TGF : transforming growth factor.

• 

  Fig. 3 Immunohistochemical staining of the intervertebral disc for IGF-1 expression. A : An IGF-1 expression was moderate in the primary group (Immuno-Peroxidase, original magnification 100). B : In the recurrent group, the chondrocytes in the intervertebral disc were stained mild by IGF-1 (Immun-Peroxidase, original magnification 100). IGF-1 : insulin-like growth factor-1.

• 

  Fig. 4 Immunohistochemical staining of the intervertebral disc for BAX. A : The chondrocytes of the intervertebral disc in primary group were stained moderately by BAX (Immun-Peroxidase, original magnification 100). B : In contrast, the chondrocytes of the intervertebral disc in the recurrent group were stained mild by BAX (Immuno-Peroxidase, original magnification 100). BAX : Bcl-2-associated X protein.

Reference

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