Restor Dent Endod.  2019 May;44(2):e17. 10.5395/rde.2019.44.e17.

Effects of the cathepsin K inhibitor with mineral trioxide aggregate cements on osteoclastic activity

• Kim HS
• Kim S
• Ko H
• Song M
• Kim M

• KMID: 2446665
• DOI: http://doi.org/10.5395/rde.2019.44.e17

Abstract

OBJECTIVES
Root resorption is an unexpected complication after replantation procedures. Combining anti-osteoclastic medicaments with retrograde root filling materials may avert this resorptive activity. The purpose of this study was to assess effects of a cathepsin K inhibitor with calcium silicate-based cements on osteoclastic activity.
METHODS
MC3T3-E1 cells were cultured for biocompatibility analyses. RAW 264.7 cells were cultured in the presence of the receptor activator of nuclear factor-kappa B and lipopolysaccharide, followed by treatment with Biodentine (BIOD) or ProRoot MTA with or without medicaments (Odanacatib [ODN], a cathepsin inhibitor and alendronate, a bisphosphonate). After drug treatment, the cell counting kit-8 assay and Alizarin red staining were performed to evaluate biocompatibility in MC3T3-E1 cells. Reverse-transcription polymerase chain reaction, tartrate-resistant acid phosphatase (TRAP) staining and enzyme-linked immunosorbent assays were performed in RAW 264.7 cells to determine the expression levels of inflammatory cytokines, interleukin (IL)-1β, IL-6, tumor necrosis factor-α (TNF-α) and prostaglandin E2 (PGE2). Data were analyzed by one-way analysis of variance and Tukey's post hoc test (p < 0.05).
RESULTS
Biocompatibility results showed that there were no significant differences among any of the groups. RAW 264.7 cells treated with BIOD and ODN showed the lowest levels of TNF-α and PGE2. Treatments with BIOD + ODN were more potent suppressors of inflammatory cytokine expression (p < 0.05).
CONCLUSION
The cathepsin K inhibitor with calcium silicate-based cement inhibits osteoclastic activity. This may have clinical application in preventing inflammatory root resorption in replanted teeth.

Keyword

Cathepsin K; Osteoclastogenesis; Osteoclast; Osteoblast; Root resorption

MeSH Terms

Acid Phosphatase
Alendronate
Calcium
Cathepsin K*
Cathepsins*
Cell Count
Cytokines
Dinoprostone
Enzyme-Linked Immunosorbent Assay
Interleukin-6
Interleukins
Miners*
Necrosis
Osteoblasts
Osteoclasts*
Pemetrexed
Polymerase Chain Reaction
RAW 264.7 Cells
Receptor Activator of Nuclear Factor-kappa B
Replantation
Root Resorption
Tooth
Acid Phosphatase
Alendronate
Calcium
Cathepsin K
Cathepsins
Cytokines
Dinoprostone
Interleukin-6
Interleukins
Pemetrexed
Receptor Activator of Nuclear Factor-kappa B