Restor Dent Endod.  2012 Nov;37(4):194-200.

Evaluation of the rat tissue reaction to experimental new resin cement and mineral trioxide aggregate cement

• Yang WK
• Ko HJ
• Kim MR

Abstract

OBJECTIVES
New resin cement (NRC) has been developed as a root repairing material and the material is composed of organic resin matrix and inorganic powders. The aim of this study was to compare the rat subcutaneous tissue response to NRC and mineral trioxide aggregate (MTA) cement and to investigate the tissue toxicity of both materials.
MATERIALS AND METHODS
Sixty rats received two polyethylene tube-implants in dorsal subcutaneous regions, MTA and NRC specimens. Twenty rats were sacrificed respectively at 1, 4 and 8 wk after implantation and sectioned to 5 microm thickness and stained with Hematoxylin-Eosin (H-E) or von-Kossa staining. The condition of tissue adjacent to the implanted materials and the extent of inflammation to each implant were evaluated by two examiners who were unaware of the type of implanted materials in the tissues. Data were statistically analyzed with paired t-test (p < 0.05).
RESULTS
In specimens implanted with both NRC and MTA, severe inflammatory reactions were present at one wk, which decreased with time. At eighth wk, MTA implanted tissue showed mild inflammatory reaction, while there were moderate inflammatory reactions in NRC implanted tissue, respectively. In NRC group, von-Kossa staining showed more calcification materials than MTA group at eighth wk.
CONCLUSIONS
It was concluded that the calcium reservoir capability of NRC may contribute to mineralization of the tissues.

Keyword

Biocompatibility; Mineral trioxide aggregate (MTA); New resin cement; Tissue reaction

MeSH Terms

Aluminum Compounds
Animals
Calcium
Calcium Compounds
Drug Combinations
Glutamates
Guanine
Inflammation
Oxides
Polyethylene
Powders
Rats
Resin Cements
Silicates
Subcutaneous Tissue
Pemetrexed
Aluminum Compounds
Calcium
Calcium Compounds
Drug Combinations
Glutamates
Guanine
Oxides
Polyethylene
Powders
Resin Cements
Silicates

Figure

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  Figure 1 The polymerization reaction formula of New resin cement (NRC).

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  Figure 2 Representative histologic characteristics of each group. (a) 1 week MTA; (b) 1 week NRC; (c) 4 weeks MTA; (d) 4 weeks NRC; (e) 8 weeks MTA; (f) 8 weeks NRC. Black arrow shows area of inflammatory reaction (H-E, ×40). MTA, mineral trioxide aggregate; NRC, new resin cement.

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  Figure 3 The histologic specimen of 1 week MTA (H-E, ×200). Black arrow shows inflammatory cells. MTA, mineral trioxide aggregate.

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  Figure 4 The histologic specimen of 1 week NRC (H-E, ×200). There are many inflammatory cells on the border of the material (Black arrow). NRC, new resin cement.

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  Figure 5 The histologic specimen of 4 weeks MTA (H-E, ×200). Calcified materials are encapsulated and surrounded by inflammatory cells (Black arrow). MTA, mineral trioxide aggregate.

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  Figure 6 The histologic specimen of 4 weeks NRC (H-E, ×200). Calcified materials are surrounded by inflammatory cells (Black arrow). NRC, new resin cement.

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  Figure 7 The histologic specimen of 8 weeks MTA (H-E, ×200). Calcified particles are surrounded with chronic inflammatory cells (Black arrow). MTA, mineral trioxide aggregate.

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  Figure 8 The histologic specimen of 8 weeks NRC (H-E, ×200). There are some inflammatory cells under the materials (Black arrow). NRC, new resin cement.

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  Figure 9 Histologic specimen of (a) 1 week MTA; (b) 1 week NRC; (c) 4 weeks MTA; (d) 4 weeks NRC; (e) 8 weeks MTA; (f) 8 weeks NRC. Black arrow shows calcified materials (von-Kossa, ×40). MTA, mineral trioxide aggregate; NRC, new resin cement.

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  Figure 10 Dystrophic calcium particles in blood vessels (8 weeks MTA, von-Kossa, ×100). MTA, mineral trioxide aggregate.

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  Figure 11 Dystrophic calcium particles in blood vessels and macrophages (8 weeks NRC, von-Kossa, ×200). NRC, new resin cement.

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