Biomol Ther.  2018 Jul;26(4):374-379. 10.4062/biomolther.2017.197.

Endothelium Independent Effect of Pelargonidin on Vasoconstriction in Rat Aorta

• Min YS
• Yoon HJ
• Je HD
• Lee JH
• Yoo SS
• Shim HS
• Lee HY
• La HO
• Sohn UD

• KMID: 2420388
• DOI: http://doi.org/10.4062/biomolther.2017.197

Abstract

In this study, we investigated the effects of pelargonidin, an anthocyanidin found in many fruits and vegetables, on endothelium-independent vascular contractility to determine the underlying mechanism of relaxation. Isometric contractions of denuded aortic muscles from male rats were recorded, and the data were combined with those obtained in western blot analysis. Pelargonidin significantly inhibited fluoride-, thromboxane A2-, and phorbol ester-induced vascular contractions, regardless of the presence or absence of endothelium, suggesting a direct effect of the compound on vascular smooth muscles via a different pathway. Pelargonidin significantly inhibited the fluoride-dependent increase in the level of myosin phosphatase target subunit 1 (MYPT1) phosphorylation at Thr-855 and the phorbol 12,13-dibutyrate-dependent increase in the level of extracellular signal-regulated kinase (ERK) 1/2 phosphorylation at Thr202/Tyr204, suggesting the inhibition of Rho-kinase and mitogen-activated protein kinase kinase (MEK) activities and subsequent phosphorylation of MYPT1 and ERK1/2. These results suggest that the relaxation effect of pelargonidin on agonist-dependent vascular contractions includes inhibition of Rho-kinase and MEK activities, independent of the endothelial function.

Keyword

ERK1/2; Fluoride; MYPT1; Pelargonidin; Phorbol ester; Rho-kinase

MeSH Terms

Animals
Anthocyanins
Aorta*
Blotting, Western
Endothelium*
Fluorides
Fruit
Humans
Isometric Contraction
Male
Muscle, Smooth, Vascular
Muscles
Myosin-Light-Chain Phosphatase
Phosphorylation
Phosphotransferases
Protein Kinases
Rats*
Relaxation
rho-Associated Kinases
Vasoconstriction*
Vegetables
Anthocyanins
Fluorides
Myosin-Light-Chain Phosphatase
Phosphotransferases
Protein Kinases
rho-Associated Kinases