Korean J Physiol Pharmacol.  2018 Mar;22(2):163-172. 10.4196/kjpp.2018.22.2.163.

Effects of salmon DNA fraction in vitro and in a monosodium iodoacetate-induced osteoarthritis rat model

Affiliations
  • 1Department of Orthopedic Surgery, Gangneung Asan Hospital, Ulsan University College of Medicine, Gangneung 25440, Korea.
  • 2Medical Research Institute, Gangneung Asan Hospital, Gangneung 25440, Korea.
  • 3Department of Pathology, Gangneung Asan Hospital, Ulsan University College of Medicine, Gangneung 25440, Korea.
  • 4Natural Products Research Institute, Korea Institute of Science and Technology, Gangneung 25440, Korea.
  • 5Department of Orthopedic Surgery, Hanil General Hospital, Seoul 01450, Korea.
  • 6Department of Surgery, Gangneung Asan Hospital, Ulsan University College of Medicine, Gangneung 25440, Korea. d990081@gmail.com

Abstract

PRF001 is a fragmented DNA polymer extracted from the testes of salmon. The purpose of this study was to assess the anti-inflammatory effect of PRF001 in vitro as well as the protective effect of PRF001 intake against arthritis in a rat model. In vitro, cell survival and inflammatory markers after H₂O₂ treatment to induce cell damage were investigated in CHON-001 cells treated with different concentrations of PRF001. In vivo, osteoarthritis was induced by intra-articular injection of monosodium iodoacetate (MIA) into the knee joints of rats. After consumption of PRF001 (10, 50, or 100 mg/kg) for 4 weeks, inflammatory mediators and cytokines in articular cartilage were investigated. In vitro, the levels of inflammatory markers, IL-1β, TNF-α, COX-2, iNOS, and PGE2, were significantly suppressed by PRF001 treatment. In vivo, the inflammatory mediators and cytokines, IL-1β, p-Erk1/2, NF-κB, TNF-α, COX-2, and PGE2, as well as MMP3 and MMP7, which have catabolic activity in chondrocytes, were decreased in the MIA-induced osteoarthritic rats following intake of PRF001. Histological analysis revealed that PRF001 had a protective effect on the articular cartilage. Altogether, these results demonstrated that the anti-inflammatory property of PRF001 contributes to its protective effects in osteoarthritis through deregulating IL-1β, TNF-α, and subsequent signals, such as p-Erk1/2, NF-κB, COX-2, PGE2, and MMPs.

Keyword

Chondrocyte; Cytokine; Inflammation; Osteoarthritis; Polydeoxyribonucleotide; PRF001

MeSH Terms

Animals
Arthritis
Cartilage, Articular
Cell Survival
Chondrocytes
Cytokines
Dinoprostone
DNA*
In Vitro Techniques*
Inflammation
Injections, Intra-Articular
Knee Joint
Matrix Metalloproteinases
Models, Animal*
Osteoarthritis*
Polymers
Rats*
Salmon*
Testis
Cytokines
DNA
Dinoprostone
Matrix Metalloproteinases
Polymers
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