Lab Anim Res.  2016 Jun;32(2):91-98. 10.5625/lar.2016.32.2.91.

Anti-inflammatory effect of egg white-chalcanthite and purple bamboo salts mixture on arthritis induced by monosodium iodoacetate in Sprague-Dawley rats

• Lee TH
• Song HK
• Jang JY
• Kim DY
• Park HK
• Choi EA
• Han BS

• KMID: 2309082
• DOI: http://doi.org/10.5625/lar.2016.32.2.91

Abstract

The aim of this study is to investigate the potential of anti-osteoarthritis effects on egg white-chalcanthite (EC), purple bamboo salts (PBS), and a mixture of EC and PBS (EC+PBS). EC is a mixture of egg white and pulverized chalcanthite. PBS has been widely used as one of functional foods in Korea and shows unique features compared with common salt. Osteoarthritis was induced by intra-articular injection of monosodium iodoacetate (MIA, 4mg/kg bw) in Sprague-Dawley (SD) rats. Test substances were administered once daily for 6 weeks at doses of 10 mg EC, EC+100 mg PBS, EC+200 mg PBS before and after MIA injection. Each substance was assessed by blood chemistry parameters, and by serum cytokines including IL-1β and IL-6, and nitric oxide (NO) and prostaglandin-E2 (PGE2). Structural changes of articular cartilage were also evaluated by histopathological examination. As a result, body weight and blood chemistry parameter were not different in all experimental groups. EC+PBS mixture reduced the production of PGE2, NO, IL-1β, and IL-6. In histological grade of osteoarthritis, EC+PBS mixture had a tendency to ameliorate damage of articular cartilage induced by MIA in a dose-dependent manner. In conclusion, EC+PBS mixture was demonstrated to have a potential for anti-inflammatory effect against osteoarthritis induced by MIA in a dose-dependent manner.

Keyword

arthritis; purple bamboo salts (PBS); egg white-chalcanthite (EC); monosodium iodoacetate (MIA)

MeSH Terms

Animals
Arthritis*
Body Weight
Cartilage, Articular
Chemistry
Cytokines
Dinoprostone
Egg White
Functional Food
Injections, Intra-Articular
Interleukin-6
Korea
Nitric Oxide
Osteoarthritis
Ovum*
Rats
Rats, Sprague-Dawley*
Salts*
Cytokines
Dinoprostone
Interleukin-6
Nitric Oxide
Salts

Figure

• 

  Figure 1 Line diagrams showing experimental design for NC, PC, EC 10, PBS 200, EC+PBS 100 and 200 treatment group in MIA treated rats. MIA: monosodium iodoacetate 4 mg/kg b.w. intra-articular injection, NC: normal control, PC: MIA alone, EC 10: egg white-chalcanthite 10 mg/kg b.w, PBS 200: purple bamboo salt 200 mg/kg b.w, EC+PBS 100: EC+PBS 100 mg/kg b.w, EC+PBS 200: EC+PBS 200 mg/kg b.w..

• 

  Figure 2 Effect of EC, PBS and EC+PBS on the body weight in rats. The data were expressed as mean±SD. NC: normal control, PC: MIA alone, EC 10: egg white-chalcanthite 10 mg/kg b.w, PBS 200: purple bamboo salt 200 mg/kg b.w, EC+PBS 100: EC+PBS 100 mg/kg b.w, EC+PBS 200: EC+PBS 200 mg/kg b.w.

• 

  Figure 3 Effects of EC, PBS and EC+PBS on concentration of nitric oxide (A) and prostaglandin E2 (B) The data were expressed as mean ± SD. The data were expressed as mean± SD. NC: Normal control, PC: MIA alone, EC 10: Egg white-chalcanthite 10 mg/kg b.w, PBS 200: Purple bamboo salt 200 mg/kg b.w, EC-PBS 100: EC+PBS 100 mg/kg b.w, EC+PBS 200: EC+PBS 200 mg/kg b.w. #, significantly different from NC at P<0.05.; *, significantly different from PC at P<0.05.

• 

  Figure 4 Effects of EC, PBS and EC+PBS on concentration of IL-1β (A) and IL-6 (B). The results were expressed as mean ± SD. The data were expressed as mean ± SD. NC: normal control, PC:MIA alone, EC 10: egg white-chalcanthite 10 mg/kg b.w, PBS 200: purple bamboo salt 200 mg/kg b.w, EC+PBS 100: EC+PBS 100 mg/kg b.w, EC+PBS 200: EC+PBS 200 mg/kg b.w. #, significantly different from NC at P<0.05; *, significantly different from PC at P<0.05.

• 

  Figure 5 Histopathological findings of articular structure (A) damaging articular structure (surface irregularity and surface cleft, arrows) of rat treated with MIA alone, H&E stain (B) damaging articular structure (matrix loss of cartilage, arrows) of rat treated with MIA alone, Safranin O stain (C) reparing articular structure of rat treated with MIA+EC+200 mg PBS, H&E stain (D) recovering articular structure of rat treated with MIA+EC+200 mg PBS, Safranin O stain.

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