Biomol Ther.  2017 May;25(3):272-278. 10.4062/biomolther.2016.098.

Protective Role of Fucoidan in Cerebral Ischemia-Reperfusion Injury through Inhibition of MAPK Signaling Pathway

• Che N
• Ma Y
• Xin Y

• KMID: 2378805
• DOI: http://doi.org/10.4062/biomolther.2016.098

Abstract

Fucoidan has been reported to exhibit various beneficial activities ranging from to antivirus and anticancer properties. However, little information is available about the effects of fucoidan on cerebral ischemia-reperfusion injury (IRI). Our study aimed to explore the effects of fucoidan on cerebral IRI, as well as the underlying mechanisms. Sprague-Dawley (SD) rats were randomly subjected to four groups: Sham, IRI+saline (IRI+S), IRI+80 mg/kg fucoidan (IRI+F80), and IRI+160 mg/kg fucoidan (IRI+F160). Fucoidan (80 mg/kg or 160 mg/kg) was intraperitoneally injected from 7 days before the rats were induced to cerebral IRI model with middle cerebral artery occlusion (MCAO) method. At 24 h after reperfusion, neurological deficits and the total infarct volume were determined. The levels of inflammation-associated cytokines (interleukin (IL)-1β, IL-6, myeloperoxidase (MPO), and tumor necrosis factor (TNF)-α), oxidative stress-related proteins (malondialdehyde (MDA) and superoxide dismutase (SOD)) in the ischemic brain were measured by enzyme-linked immunosorbent assay (ELISA). Besides, the levels of apoptosis-related proteins (p-53, Bax, and B-cell lymphoma (Bcl)-2) and mitogen-activated protein kinase (MAPK) pathway (phosphorylation-extracellular signal-regulated kinase (p-ERK), p-c-Jun N-terminal kinase (JNK), and p-p38) were measured. Results showed that administration of fucoidan significantly reduced the neurological deficits and infarct volume compared to the IRI+S group in a dose-dependent manner. Also, fucoidan statistically decreased the levels of inflammation-associated cytokines, and oxidative stress-related proteins, inhibited apoptosis, and suppressed the MAPK pathway. So, Fucoidan plays a protective role in cerebral IRI might be by inhibition of MAPK pathway.

Keyword

Fucoidan; Cerebral ischemia-reperfusion injury; MAPK pathway

MeSH Terms

Animals
Apoptosis
Brain
Cytokines
Enzyme-Linked Immunosorbent Assay
Infarction, Middle Cerebral Artery
Interleukin-6
Lymphoma, B-Cell
Methods
Peroxidase
Phosphotransferases
Protein Kinases
Rats
Rats, Sprague-Dawley
Reperfusion
Reperfusion Injury*
Superoxide Dismutase
Tumor Necrosis Factor-alpha
Cytokines
Interleukin-6
Peroxidase
Phosphotransferases
Protein Kinases
Superoxide Dismutase
Tumor Necrosis Factor-alpha