J Korean Med Sci.  2000 Dec;15(6):627-630. 10.3346/jkms.2000.15.6.627.

Effectiveness of propofol pretreatment on the extent of deranged cerebral mitochondrial oxidative enzyme system after incomplete forebrain ischemia/reperfusion in rats

• Lee Y
• Chung C
• Oh YS

• KMID: 2157541
• DOI: http://doi.org/10.3346/jkms.2000.15.6.627

Abstract

It has been suggested that propofol has the protective effect on cerebral ischemia-reperfusion injury. The aim of this study is to evaluate the effect of propofol pretreatment on incomplete forebrain ischemia-reperfusion injury in rats. Thirty Sprague-Dawley rats were anesthetized with isoflurane in oxygen and randomly allocated into propofol group (n=13) and saline group (n=17). In propofol group, propofol was pretreated in a step-down scheme before inducing forebrain ischemia by occlusion of both common carotid arteries and arterial hypotension. After ischemia (20 min) and reperfusion (30 min), rats were decapitated. Brain was sliced to obtain coronal slices of 4-12 mm from frontal pole, which were reacted with 2% 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) for 10 min to differentiate the damaged tissues from normal tissues. Median (interquartile range) values of the average percent infarct area were 0.0 (8.6)% and 20.1 (41.2)% in propofol and saline groups, respectively. There was significant difference between the groups. In conclusion, propofol may have a protective effect on incomplete forebrain ischemia-reperfusion injury.

Keyword

Brain Ischemia; Propofol; Reperfusion Injury; Tetrazolium Salts

MeSH Terms

Animal
Brain Ischemia/prevention & control*
Brain Ischemia/pathology
Cerebral Infarction/prevention & control
Cerebral Infarction/pathology
Disease Models, Animal
Free Radical Scavengers/pharmacology*
Mitochondria/enzymology*
Neuroprotective Agents/pharmacology*
Oxidative Phosphorylation
Propofol/pharmacology*
Prosencephalon/metabolism
Prosencephalon/injuries
Prosencephalon/drug effects*
Rats
Rats, Sprague-Dawley
Reperfusion Injury/prevention & control*
Reperfusion Injury/pathology
Tetrazolium Salts