J Korean Soc Emerg Med.  1999 Jun;10(2):175-182.

The Relationship between Cerebral Reperfusion Flow and the Ischemic Histopathologic Damage after Incomplete Forebrain Ischemia in Rat Model

• Hwang TS
• Seo JP
• Song KJ
• Jeong YK
• Shin BH
• Kim SH

Abstract

BACKGROUND: Experimental data indicate that low-flow reperfusion following prolonged cardiocirculatory arrest may aggravate early cerebral microcirculatory repefusion disorders. We investigated the influence of cerebral repefusion flow change to the ischemic histopathologic damage of brain tissue after incomplete forebrain ischemia in rats. MATERIALS AND METHOD: Anesthetized Sprague-Dawley rats were undergone ligation of both infernal carotid artery by microvascular clamp for 10 minutes. After release of the clamp, reperfusion was started with several different flow levels (0, 10, 20, 30, 50, and 100%) of infernal carotid artery comparing to pre-clamping phase using flowmeter. After 15minutes of reperfusion, rat brains were prepared by perfusion-fixation with 3% formaldehyde. Under light microscopic examination of Hematoxylin-Eosin stained tissue slide, histopathologic damage was examined at cortex, putamen, and hippocampus regions. Categorical hisotopathologic damage scores were derived in each regions by manual counts of ischemic neurons. RESULT: The histopathologic damage scores were 0, 10. 2+/-0.5, 7.6+/-1.5, 5.9+/-1.4, 5.0+/- 2.8, 3.5+/-0.7, and 1.0+/-0.0 in control, 0, 10, 20, 30, 50, and 100% reperfusion groups, respectively(p<0.05).
CONCLUSION
Our insults showed significant increment of brain histopathologic damage scores along with decreasing amount of cerebral reperfusion know after incomplete forebrain ischemia. We believe restoration of repefusion flow to pre-ischemic level would be a critical component in attenuation of brain ischemic damage.

MeSH Terms

Animals
Brain
Carotid Arteries
Flowmeters
Formaldehyde
Hippocampus
Ischemia*
Ligation
Models, Animal*
Neurons
Prosencephalon*
Putamen
Rats*
Rats, Sprague-Dawley
Reperfusion*
Formaldehyde