J Cancer Prev.
2016 Dec;21(4):257-263. 10.15430/JCP.2016.21.4.257.
Protective Effect of an Isoflavone, Tectorigenin, Against Oxidative Stress-induced Cell Death via Catalase Activation
- Affiliations
-
- 1Department of Biochemistry, Jeju National University School of Medicine, Jeju, Korea. jinwonh@jejunu.ac.kr
- 2School of Medical Science and Laboratory Medicine, Jiangsu University, Jiangsu, China.
- 3Department of Microbial Chemistry, College of Pharmacy, Kyung Hee University, Seoul, Korea.
- 4Radiation Biotechnology Research Division, Korea Atomic Energy Research Institute, Jeongeup, Korea.
- 5Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute, Daejeon, Korea.
- 6Department of Radiation Biotechnology and Applied Radioisotope, Korea University of Science and Technology, Daejeon, Korea.
- KMID: 2367563
- DOI: http://doi.org/10.15430/JCP.2016.21.4.257
Abstract
- BACKGROUND
Isoflavones are biologically active compounds that occur naturally in a variety of plants, with relatively high levels in soybean. Tectorigenin, an isoflavone, protects against hydrogen peroxide (H2O2)-induced cell damage. However, the underlying mechanism is unknown.
METHODS
The MTT assay was performed to determine cell viability. Catalase activity was assessed by determining the amount of enzyme required to degrade 1 μM H2O2. Protein expression of catalase, phospho-extracellular signal-regulated kinase (ERK), IκB-α, and NF-κB were evaluated by Western blot analysis. A mobility shift assay was performed to assess the DNA-binding ability of NF-κB. Transient transfection and a NF-κB luciferase assay were performed to assess transcriptional activity.
RESULTS
Tectorigenin reduced H2O2-induced death of Chinese hamster lung fibroblasts (V79-4). In addition, tectorigenin increased the activity and protein expression of catalase. Blockade of catalase activity attenuated the protective effect of tectorigenin against oxidative stress. Furthermore, tectorigenin enhanced phosphorylation of ERK and nuclear expression of NF-κB, while inhibition of ERK and NF-κB attenuated the protective effect of tectorigenin against oxidative stress.
CONCLUSIONS
Tectorigenin protects cells against oxidative damage by activating catalase and modulating the ERK and NF-κB signaling pathway.
MeSH Terms
-
Animals
Blotting, Western
Catalase*
Cell Death*
Cell Survival
Cricetinae
Cricetulus
Electrophoretic Mobility Shift Assay
Extracellular Signal-Regulated MAP Kinases
Fibroblasts
Hydrogen Peroxide
Isoflavones
Luciferases
Lung
NF-kappa B
Oxidative Stress
Phosphorylation
Phosphotransferases
Soybeans
Transfection
Catalase
Extracellular Signal-Regulated MAP Kinases
Hydrogen Peroxide
Isoflavones
Luciferases
NF-kappa B
Phosphotransferases
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