Tissue Eng Regen Med.  2016 Dec;13(6):701-712. 10.1007/10.1007/s13770-016-0018-x.

Immunomodulatory Effects of Adipose Tissue-Derived Stem Cells on Elastin Scaffold Remodeling in Diabetes

• Chow JP
• Simionescu DT
• Carter AL
• Simionescu A

• KMID: 2360882
• DOI: http://doi.org/10.1007/10.1007/s13770-016-0018-x

Abstract

Diabetes is a major risk factor for the progression of vascular disease, contributing to elevated levels of glycoxidation, chronic inflammation and calcification. Tissue engineering emerges as a potential solution for the treatment of vascular diseases however there is a considerable gap in the understanding of how scaffolds and stem cells will perform in patients with diabetes. We hypothesized that adipose tissue-derived stem cells (ASCs) by virtue of their immunosuppressive potential would moderate the diabetes-intensified inflammatory reactions and induce positive construct remodeling. To test this hypothesis, we prepared arterial elastin scaffolds seeded with autologous ASCs and implanted them subdermally in diabetic rats and compared inflammatory markers, macrophage polarization, matrix remodeling, calcification and bone protein expression to control scaffolds implanted with and without cells in nondiabetic rats. ASC-seeded scaffolds exhibited lower levels of CD8+ T-cells and CD68+ pan-macrophages and higher numbers of M2 macrophages, smooth muscle cell-like and fibroblast-like cells. Calcification and osteogenic markers were reduced in ASCseeded scaffolds implanted in non-diabetic rats but remained unchanged in diabetes, unless the scaffolds were first pre-treated with penta-galloyl glucose (PGG), a known anti-oxidative elastin-binding polyphenol. In conclusion, autologous ASC seeding in elastin scaffolds is effective in combating diabetes-related complications. To prevent calcification, the oxidative milieu needs to be reduced by elastin-binding antioxidants such as PGG.

Keyword

Streptozotocin-induced diabetes; Arterial scaffolds; Macrophage polarization; Calcification

MeSH Terms

Animals
Antioxidants
Diabetes Complications
Elastin*
Glucose
Humans
Inflammation
Macrophages
Muscle, Smooth
Prostaglandins G
Rats
Risk Factors
Stem Cells*
T-Lymphocytes
Tissue Engineering
Vascular Diseases
Virtues
Antioxidants
Elastin
Glucose
Prostaglandins G