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The Effect of Cryopreservation of Allograft Nerve on the Recovery of Motor Function

Choi SS, Park I, Hyun JK, Jung JU, Kim JP

PURPOSE: Peripheral nerve allograft can be an acceptable alternative, but it has not yet become clinically useful because of immune response to foreign tissue. With significant advances in the research...
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Overview of Urethral Reconstruction by Tissue Engineering: Current Strategies, Clinical Status and Future Direction

Rashidbenam Z, Jasman MH, Hafez P, Tan GH, Goh EH, Fam XI, Ho CC, Zainuddin , Rajan R, Mohd Nor F, Shuhaili M, Kosai NR, Imran FH, Ng MH

BACKGROUND: Urinary tract is subjected to a variety of disorders such as urethral stricture, which often develops as a result of scarring process. Urethral stricture can be treated by urethral...
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Current Trends in Viral Gene Therapy for Human Orthopaedic Regenerative Medicine

Venkatesan JK, Rey-Rico A, Cucchiarini M

BACKGROUND: Viral vector-based therapeutic gene therapy is a potent strategy to enhance the intrinsic reparative abilities of human orthopaedic tissues. However, clinical application of viral gene transfer remains hindered by...
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Angiotensin II and TGF-β1 Induce Alterations in Human Amniotic Fluid-Derived Mesenchymal Stem Cells Leading to Cardiomyogenic Differentiation Initiation

Gasiūnienė M, Petkus G, Matuzevičius D, Navakauskas D, Navakauskienė R

BACKGROUND AND OBJECTIVES: Human amniotic fluid-derived mesenchymal stem cells (AF-MSCs) may be a valuable source for cardiovascular tissue engineering and cell therapy. The aim of this study is to verify...
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FxClear, A Free-hydrogel Electrophoretic Tissue Clearing Method for Rapid De-lipidation of Tissues with High Preservation of Immunoreactivity

Choi J, Lee E, Kim JH, Sun W

Over the last two decades, several tissue clearing methodologies have been established that render tissues optically transparent and allow imaging of unsectioned tissues of significant volumes, thus improving the capacity...
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Matrix Production in Chondrocytes Transfected with Sex Determining Region Y-Box 9 and Telomerase Reverse Transcriptase Genes: An In Vitro Evaluation from Monolayer Culture to Three-Dimensional Culture

Noorhidayah MN, Zulkifly A, Khalid KA, Zainol I, Zamli Z, Sha'ban M

BACKGROUND: This study aimed to observe the cartilaginous matrix production in SRY (sex determining region Y)-box 9 (SOX9)- and/or telomerase reverse transcriptase (TERT)-transfected chondrocytes from monolayer to three-dimensional (3D) culture. METHODS:...
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Histological Method to Study the Effect of Shear Stress on Cell Proliferation and Tissue Morphology in a Bioreactor

Chabanon M, Duval H, Grenier J, Beauchesne C, Goyeau B, David B

BACKGROUND: Tissue engineering represents a promising approach for the production of bone substitutes. The use of perfusion bioreactors for the culture of bone-forming cells on a three-dimensional porous scaffold resolves...
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Development of a mouse model for pulp-dentin complex regeneration research: a preliminary study

Kim S, Lee S, Jung HS, Kim SY, Kim E

OBJECTIVES: To achieve pulp-dentin complex regeneration with tissue engineering, treatment efficacies and safeties should be evaluated using in vivo orthotopic transplantation in a sufficient number of animals. Mice have been...
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Stem Cells Seeded on Multilayered Scaffolds Implanted into an Injured Bladder Rat Model Improves Bladder Function

Shrestha K, Jeon SH, Jung AR, Kim IG, Kim GE, Park YH, Kim SH, Lee JY

BACKGROUND: To investigate whether human adipose-derived stem cells (hADSCs) seeded on multilayered poly (L-lactide-co-ε-caprolactone) (PLCL) sheets improve bladder function in a rat model of detrusor smooth muscle-removed bladder. METHODS: Male rats...
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Proliferation Profile of Uterine Endometrial Stromal Cells during In Vitro Culture with Gonadotropins: Recombinant versus Urinary Follicle Stimulating Hormone

Kim YJ, Kim YY, Song DY, Lee SH, Park CW, Kim H, Ku SY

BACKGROUND: Provision of optimal endometrial stromal cells is essential in uterine tissue engineering. Culture of these cells is significantly influenced by gonadotropin hormones. This investigation attempted to define the proliferation...
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A Comparative Study of the Effects of Different Decellularization Methods and Genipin-Cross-Linking on the Properties of Tracheal Matrices

Zhong Y, Jiang A, Sun F, Xiao Y, Gu Y, Wu L, Zhang Y, Shi H

BACKGROUND: Different decellularization methods can affect the integrity and the biomechanical and biocompatible properties of the tracheal matrix. Natural cross-linking with genipin can be applied to improve those properties. The...
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Hybrid Additive Microfabrication Scaffold Incorporated with Highly Aligned Nanofibers for Musculoskeletal Tissues

Sooriyaarachchi D, Minière H, Maharubin , Tan GZ

BACKGROUND: Latest tissue engineering strategies for musculoskeletal tissues regeneration focus on creating a biomimetic microenvironment closely resembling the natural topology of extracellular matrix. This paper presents a novel musculoskeletal tissue...
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Tissue Regeneration of Human Mesenchymal Stem Cells on Porous Gelatin Micro-Carriers by Long-Term Dynamic In Vitro Culture

Nguyen L, Bang S, Noh I

BACKGROUND: Tissue engineering is a multidisciplinary field which attracted much attention in recent years. One of the most important issue in tissue engineering is how to obtain high cell numbers...
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Decellularization of Trachea With Combined Techniques for Tissue-Engineered Trachea Transplantation

Batioglu-Karaaltin A, Ovali E, Karaaltin , Yener M, Yılmaz M, Eyüpoğlu F, Yılmaz YZ, Bozkurt ER, Demir N, Konuk E, Bozdağ ES, Yiğit O, Cansiz H

OBJECTIVES: The purpose of this study is to shorten the decellularization time of trachea by using combination of physical, chemical, and enzymatic techniques. METHODS: Approximately 3.5-cm-long tracheal segments from 42 New...
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3D architecture developments for spatial controls of periodontal ligament regeneration with angular orientations

Park CH

Various biomaterial-based, 3D architectures with micron-scaled geometries have been investigated for periodontal tissue neogenesis, and appropriate fabrication techniques for the scaffold manufacturing have been developed for pre-clinical or clinical situations....
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Development of Artificial Substitutes for Upper Digestive Tract: Personal Experience of Trials and Errors

Chung EJ

The current treatments for esophageal diseases, such as carcinomas, trauma or congenital malformations, require surgical intervention, and esophageal reconstruction using redundant parts of the gastrointestinal tract. However, the use of...
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Study of Recellularized Human Acellular Arterial Matrix Repairs Porcine Biliary Segmental Defects

Liu W, Zhang SN, Hu ZQ, Feng SM, Li ZH, Xiao SF, Wang HS, Li L

BACKGROUND: With the popularity of laparoscopic cholecystectomy, common bile duct injury has been reported more frequently. There is no perfect method for repairing porcine biliary segmental defects. METHODS: After the decellularization...
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Evaluation of Polycaprolactone-Associated Human Nasal Chondrocytes as a Therapeutic Agent for Cartilage Repair

Kim DH, Lim MH, Jeun JH, Park SH, Lee W, Park SH, Kwon MY, Hwang SH, Kim SW

BACKGROUND: In this study, we manufactured a complex of human nasal septal cartilage (hNC) with polycaprolactone (PCL) for transplantation into cartilaginous skeletal defects and evaluated their characteristics. METHODS: Nasal septum tissue...
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Development of Macroporous Chitosan Scaffolds for Eyelid Tarsus Tissue Engineering

Sun MT, O'Connor A, Milne I, Biswas D, Casson R, Wood J, Selva D

BACKGROUND: Reconstruction of large eyelid defects remains challenging due to the lack of suitable eyelid tarsus tissue substitutes. We aimed to evaluate a novel bioengineered chitosan scaffold for use as...
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Current Progress in Tendon and Ligament Tissue Engineering

Lim WL, Liau LL, Ng MH, Chowdhury , Law JX

BACKGROUND: Tendon and ligament injuries accounted for 30% of all musculoskeletal consultations with 4 million new incidences worldwide each year and thus imposed a significant burden to the society and...
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