Int J Stem Cells.  2015 Nov;8(2):219-227. 10.15283/ijsc.2015.8.2.219.

Fibrin Scaffolds Designing in order to Human Adipose-derived Mesenchymal Stem Cells Differentiation to Chondrocytes in the Presence of TGF-beta3

Affiliations
  • 1Stem Cell laboratory, The Academic Center for Education, Culture and Research, Qom Branch, Qom, Iran. m.ghiasi@acecr.ac.ir

Abstract

BACKGROUND AND OBJECTIVES
One of the most cellular source used for cartilage tissue engineering are mesenchymal stem cells (MSCs). In present study, human MSCs were used as cellular source. Since scaffold plays an important role in tissue engineering the aim of this study is to assess fibrin scaffold ability in chondrogenic differentiation of adipose-derived mesenchymal stem cells (ADMSCs).
METHODS
ADMSCs were isolated and cultured in DMEM medium supplemented with 10% FBS. Also ADMSCs expanded and characterised by flow cytometry. ADMSCs expressed CD44, CD90, CD105 but not CD34. After trypsinization, cells were entered within the fibrin scaffold. Then, chondrogenic medium was added to the scaffold. Seven days after cell culture, cell viability and proliferation were assessed by MTT test. Finally, 14 days after the ending of chondrogenic differentiation, analysis of chondrogenic genes expression was evaluated by RT-PCR and Real time PCR. Also, formation and development of chondrocyte cells was analysed by histological and immunohistochemistry evaluations.
RESULTS
Viability and proliferation as well as chondrogenic genes expression within fibrin scaffold increased significantly compared with control group (cells free scaffold). Also, histological and immunohistochemistry evaluation showed that chondrocyte cells and collagen type II are formed on fibrin scaffold.
CONCLUSIONS
Fibrin is a suitable scaffold for chondrogenic differentiation of ADMSCs.

Keyword

Tissue Engineering; Adipose-Derived Mesenchymal Stem Cells (ADMSCs); Chondrogenic Differentiation; Fibrin

MeSH Terms

Cartilage
Cell Culture Techniques
Cell Survival
Chondrocytes*
Collagen Type II
Fibrin*
Flow Cytometry
Humans*
Immunohistochemistry
Mesenchymal Stromal Cells*
Real-Time Polymerase Chain Reaction
Tissue Engineering
Transforming Growth Factor beta3*
Trypsin
Collagen Type II
Fibrin
Transforming Growth Factor beta3
Trypsin
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