Biomol Ther.  2016 Jul;24(4):387-394. 10.4062/biomolther.2015.176.

Trichostatin A Protects Liver against Septic Injury through Inhibiting Toll-Like Receptor Signaling

• Kim SJ
• Park JS
• Lee DW
• Lee SM

• KMID: 2351687
• DOI: http://doi.org/10.4062/biomolther.2015.176

Abstract

Sepsis, a serious clinical problem, is characterized by a systemic inflammatory response to infection and leads to organ failure. Toll-like receptor (TLR) signaling is intimately implicated in hyper-inflammatory responses and tissue injury during sepsis. Histone deacetylase (HDAC) inhibitors have been reported to exhibit anti-inflammatory properties. The aim of this study was to investigate the hepatoprotective mechanisms of trichostatin A (TSA), a HDAC inhibitor, associated with TLR signaling pathway during sepsis. The anti-inflammatory properties of TSA were assayed in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Polymicrobial sepsis was induced in mice by cecal ligation and puncture (CLP), a clinically relevant model of sepsis. The mice were intraperitoneally received TSA (1, 2 or 5 mg/kg) 30 min before CLP. The serum and liver samples were collected 6 and 24-h after CLP. TSA inhibited the increased production of tumor necrosis factor (TNF)-α and interleukin (IL)-6 in LPS-stimulated RAW264.7 cells. TSA improved sepsis-induced mortality, attenuated liver injury and decreased serum TNF-α and IL-6 levels. CLP increased the levels of TLR4, TLR2 and myeloid differentiation primary response protein 88 (MyD88) protein expression and association of MyD88 with TLR4 and TLR2, which were attenuated by TSA. CLP increased nuclear translocation of nuclear factor kappa B and decreased cytosolic inhibitor of kappa B (IκB) protein expression, which were attenuated by TSA. Moreover, CLP decreased acetylation of IκB kinase (IKK) and increased association of IKK with IκB and TSA attenuated these alterations. Our findings suggest that TSA attenuates liver injury by inhibiting TLR-mediated inflammatory response during sepsis.

Keyword

Histone deacetylase inhibitor; Nuclear factor kappa B; Sepsis; Toll-like receptor; Trichostatin A

MeSH Terms

Acetylation
Animals
Cytosol
Histone Deacetylase Inhibitors
Histone Deacetylases
Interleukin-6
Interleukins
Ligation
Liver*
Mice
Mortality
NF-kappa B
Phosphotransferases
Punctures
Sepsis
Toll-Like Receptors*
Tumor Necrosis Factor-alpha
Histone Deacetylase Inhibitors
Histone Deacetylases
Interleukin-6
Interleukins
NF-kappa B
Phosphotransferases
Toll-Like Receptors
Tumor Necrosis Factor-alpha