Mycobiology.  2011 Mar;39(1):12-19.

Assessment of Antioxidant and Phenolic Compound Concentrations as well as Xanthine Oxidase and Tyrosinase Inhibitory Properties of Different Extracts of Pleurotus citrinopileatus Fruiting Bodies

• Alam N
• Yoon KN
• Lee KR
• Kim HY
• Shin PG
• Cheong JC
• Yoo YB
• Shim MJ
• Lee MW
• Lee TS

Abstract

Cellular damage caused by reactive oxygen species has been implicated in several diseases, thus establishing a significant role for antioxidants in maintaining human health. Acetone, methanol, and hot water extracts of Pleurotus citrinopileatus were evaluated for their antioxidant activities against beta-carotene-linoleic acid and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, reducing power, ferrous ion-chelating abilities, and xanthine oxidase inhibitory activities. In addition, the tyrosinase inhibitory effects and phenolic compound contents of the extracts were also analyzed. Methanol and acetone extracts of P. citrinopileatus showed stronger inhibition of beta-carotene-linoleic acid compared to the hot water extract. Methanol extract (8 mg/mL) showed a significantly high reducing power of 2.92 compared to the other extracts. The hot water extract was more effective than the acetone and methanole extracts for scavenging DPPH radicals. The strongest chelating effect (92.72%) was obtained with 1.0 mg/mL of acetone extract. High performance liquid chromatography analysis detected eight phenolic compounds, including gallic acid, protocatechuic acid, chlorogenic acid, ferulic acid, naringenin, hesperetin, formononetin, and biochanin-A, in an acetonitrile and hydrochloric acid (5 : 1) solvent extract. Xanthine oxidase and tyrosinase inhibitory activities of the acetone, methanol, and hot water extracts increased with increasing concentration. This study suggests that fruiting bodies of P. citrinopileatus can potentially be used as a readily accessible source of natural antioxidants.

Keyword

Antioxidant; Phenolic compounds; Pleurotus citrinopileatus; Tyrosinase inhibition; Xanthine oxidase

MeSH Terms

Acetone
Acetonitriles
Antioxidants
Biphenyl Compounds
Chlorogenic Acid
Chromatography, Liquid
Coumaric Acids
Flavanones
Fruit
Gallic Acid
Hesperidin
Humans
Hydrochloric Acid
Hydroxybenzoates
Isoflavones
Methanol
Monophenol Monooxygenase
Phenol
Picrates
Pleurotus
Reactive Oxygen Species
Water
Xanthine
Xanthine Oxidase
Acetone
Acetonitriles
Antioxidants
Biphenyl Compounds
Chlorogenic Acid
Coumaric Acids
Flavanones
Gallic Acid
Hesperidin
Hydrochloric Acid
Hydroxybenzoates
Isoflavones
Methanol
Monophenol Monooxygenase
Phenol
Picrates
Reactive Oxygen Species
Water
Xanthine
Xanthine Oxidase