Mycobiology.  2014 Sep;42(3):296-300. 10.5941/MYCO.2014.42.3.296.

Characterization of an Anti-gout Xanthine Oxidase Inhibitor from Pleurotus ostreatus

• Jang IT
• Hyun SH
• Shin JW
• Lee YH
• Ji JH
• Lee JS

• KMID: 2312763
• DOI: http://doi.org/10.5941/MYCO.2014.42.3.296

Abstract

We selected Pleurotus ostreatus from among several edible mushrooms because it has high anti-gout xanthine oxidase (XOD) inhibitory activity. The maximal amount of XOD inhibitor was extracted when the Pleurotus ostreatus fruiting body was treated with distilled water at 40degrees C for 48 hr. The XOD inhibitor thus obtained was purified by Sephadex G-50 gel permeation chromatography, ultrafiltration, C18 solid phase extraction chromatography and reverse-phase high-performance liquid chromatography with 3% of solid yield, and its XOD inhibitory activity was 0.9 mg/mL of IC50. The purified XOD inhibitor was a tripeptide with the amino acid sequence phenylalanine-cysteine-histidine and a molecular weight of 441.3 Da. The XOD inhibitor-containing ultrafiltrates from Pleurotus ostreatus demonstrated dose-dependent anti-gout effects in a Sprague-Dawley rat model of potassium oxonate-induced gout, as shown by decreased serum urated levels at doses of 500 and 1,000 mg/kg, although the effect was not as great as that achieved with the commercial anti-gout agent, allopurinol when administered at a dose of 50 mg/kg.

Keyword

Anti-gout xanthine oxidase inhibitor; Edible oyster mushroom; Pleurotus ostreatus

MeSH Terms

Agaricales
Allopurinol
Amino Acid Sequence
Chromatography
Chromatography, Gel
Chromatography, Liquid
Fruit
Gout
Inhibitory Concentration 50
Models, Animal
Molecular Weight
Pleurotus*
Potassium
Rats, Sprague-Dawley
Solid Phase Extraction
Ultrafiltration
Water
Xanthine Oxidase*
Allopurinol
Potassium
Water
Xanthine Oxidase