Korean J Obstet Gynecol.  2001 Dec;44(12):2194-2200.

Cell Cycle and Gene Expression of Adriamycin-Induced Apoptosis in Human Cervical Carcinoma HeLa cells

• Kim JH
• Lee TH
• Hwangbo HJ
• Lee YH
• Koh MW

Abstract

OBJECTIVE
This work was demonstrated the induction of apoptosis in response to adriamycin, we checked the cell cycle of adriamycin-induced apoptosis and to investigate whether differential expression is associated with adriamycin-induced genes in human cervical carcinoma HeLa cells.
METHODS
Apoptosis was measured by flow cytometry for cell cycle analysis in Hela cells. Differential expression is associated with adriamycin-induced genes in HeLa cells, it was performed to purifiy the RNA, cDNA probe and hybridization. The various different overexpressed genes were determined by gene array analysis (GDA). Analysis were referenced Incyte Genomics Co. (http://www.ncbi.nlm.nih.gov/).
RESULTS
We found that adriamycin was induced apoptosis in a dose- and time-dependent manner, as demonstrated by sub-G0/G1 peaks in DNA content histogram of cell cycle. The cells of G2/M phase by treatment of 0.1 microgram/mL adriamycin had been arrested. G2/M peaks in DNA content was decreased in a dose and time-dependent manner. It had been observed 6 group, 16 genes. The group I contained thioredoxin and cytochrome c oxidase subunit IV gene, group II were p53 and excision repair protein (ERCC-1) gene. Group III was metabolic regulated gene, glucosidase, AMP deaminase isoform L (AMPD2), glutamine synthetase, cholesterol 25-hydroxylase, and steryl-sulfatase precursor. Group IV was cell skeleton constructed gene, heparan sulfate proteoglycan (HSPG2), and microfibrillar-associated protein (MFAP2), group V was oncogene group, v-yes-1 Yamaguchi sarcoma viral oncogene homolog-1 (YES1) and tyrosine kinase ELK1. The other group 6 contained NOD1 protein gene interleukine-1 receptor accessory protein (IL1RAP), pregnancy-specific glycoprotein-11 (PSG11), and pregnancy-specific protein-1a (PSG-1a).
CONCLUSION
The present findings indicating that adriamycin was revealed apoptosis in Hela cell. Differential gene expression is related in various metabolism by adriamycin.

Keyword

HeLa cell; Adriamycin; Apoptosis; Cell cycle; Gene array

MeSH Terms

AMP Deaminase
Apoptosis*
Cell Cycle*
Cholesterol
DNA
DNA Repair
DNA, Complementary
Doxorubicin
Electron Transport Complex IV
Flow Cytometry
Gene Expression*
Genomics
Glucosidases
Glutamate-Ammonia Ligase
HeLa Cells*
Heparan Sulfate Proteoglycans
Humans*
Metabolism
Oncogenes
Protein-Tyrosine Kinases
RNA
Sarcoma
Skeleton
Steryl-Sulfatase
Thioredoxins
AMP Deaminase
Cholesterol
DNA
DNA, Complementary
Doxorubicin
Electron Transport Complex IV
Glucosidases
Glutamate-Ammonia Ligase
Heparan Sulfate Proteoglycans
Protein-Tyrosine Kinases
RNA
Steryl-Sulfatase
Thioredoxins