Korean J Nutr.
2008 Jan;41(1):13-21.
Indol-3-Carbinol Regulated Tight Junction Permeability and Associated-Protein Level and Suppressed Cell Invasion in Human Colon Cancer Cell Line, HT-29
- Affiliations
-
- 1Department of Pharmaceutical Science, University of Maryland, School of Pharmacy, Baltimore, MD 21201, USA.
- 2Department of Biochemistry, Dongeui University, College of Oriental Medicine, Busan 614-052, Korea.
- 3Department of Food and Nutrition, Gimcheon College, Gimcheon 740-704, Korea. wkchoe@gmail.com
Abstract
- To determine whether indol-3-carbinol (I3C, C9H9NO), an autolysis product of a glucosinolate and a glucobrassicin in vegetables, regulated tight junction proteins (TJ) and suppressed cell invasion in colon cancer cells,this experiment was performed. Our results indicate that I3C inhibit cell growth of HT-29 cells in a dose (0, 50, 100 micrometer) and time (0, 24 and 48 h) dependent manner. Using the wound healing and matrigel invasion study, respectively, I3C inhibits the cell motility and invasion of the ovarian cancer cell line. The TEER values were increased in HT-29 cells grown in transwells treated with I3C, reversely, paracellular permeability was decreased in those of condition. Claudin-1, claudin-5, ZO-1 and occuldin have been shown to be positively expressed in HT-29 coloncancer cells. I3C occurs concurrently with a significant decrease in the levels of those of proteins in HT-29 cells. But E-cadherin level in the HT-29 was increased by I3C. The reduction of claudin-1 and claudin-5 protein levels occurred post-transcriptionaly since their mRNA levels are no difference by I3C. Therefore, our results suggest that I3C may be expected to inhibit cancer metastasis and invasion by tighten the cell junction and restoring tight junction in colon cancer cell line, HT-29.
Keyword
MeSH Terms
-
Autolysis
Cadherins
Cell Line
Cell Movement
Claudin-1
Claudin-5
Collagen
Colon
Colonic Neoplasms
Drug Combinations
Glucosinolates
HT29 Cells
Humans
Indoles
Intercellular Junctions
Laminin
Neoplasm Metastasis
Ovarian Neoplasms
Permeability
Proteins
Proteoglycans
RNA, Messenger
Tight Junction Proteins
Tight Junctions
Vegetables
Wound Healing
Cadherins
Claudin-1
Claudin-5
Collagen
Drug Combinations
Glucosinolates
Indoles
Laminin
Proteins
Proteoglycans
RNA, Messenger
Tight Junction Proteins
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Effect of Indole-3-Carbinol on Inhibition of MMP Activity via MAPK Signaling Pathway in Human Prostate Cancer Cell Line, PC3 Cells
- Effect of Inonotus Obliques Extracts on Proliferation and Caspase-3 Activity in Human Castro-Intestinal Cancer Cell Lines
- Effect of Interferon-r on Fas-mediated Apoptosis of Colon Cancer Cell Line HT-29
- The Inhibitory Effect of Curcumin on the Growth of Human Colon Cancer Cells (HT-29, WiDr) in vitro
- Changes of Tight Junction and Epithelial Cells after Treatment of Cadmium
