Korean J Nutr.  2003 Jan;36(1):18-23.

Effect of Inonotus Obliques Extracts on Proliferation and Caspase-3 Activity in Human Castro-Intestinal Cancer Cell Lines

Affiliations
  • 1Department of Food and Nutrition, Kookmin University, Seoul, Korea.
  • 2Seoul Allergy Research Center, Seoul, Korea.

Abstract

We studied the effects of hot water extract of Inonotus obliquos mushroom on the proliferation and apoptosis of the human colon adenocarcinoma, HT-29 and the human stomach adenocarcinoma, SNU-484 cell. Cells were maintained with Dulbecco's modified Eagle medium/Ham's F-12 nutrient mixture supplemented with 10% fetal bovine serum at 37 degrees C in a humidified CO2. For the cell proliferation experiments, cells were seeded in 35 mm dishes, and were treated with the various concentrations of the extract for the different time course. Apoptosis was measured by caspase-3 activity. When we incubated HT-29 cells for 24, 48, 72, and 96 hours after treatments, the cell proliferation was more suppressed with more treatment time. In case of the human stomach cancer cell, SNU484, the extract significantly decreased the cell number. Thus, the treatment of 1.5 mg/ml extract decreased almost half of the cell number. Caspase-3 activity in HT-29 was increased by the treatment of mushroom extracts. In SNU484, caspase-3 activity tended to increase in proportion to the amounts of the extracts and the treatment of Inonotus obliquos affected the activity a lot. Therefore, Inonotus obliquos is suggested for the prevention of gastro-intestinal cancer and strongly recommended for the treatment of stomach cancer.

Keyword

apoptosis; caspase-3 activity; Inonotus obliquos; cancer cell

MeSH Terms

Adenocarcinoma
Agaricales
Apoptosis
Caspase 3*
Cell Count
Cell Line*
Cell Proliferation
Colon
Eagles
HT29 Cells
Humans*
Stomach
Stomach Neoplasms
Water
Caspase 3
Water
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