J Korean Orthop Res Soc.  1999 Apr;2(1):11-17.

Cytokine Response of the Synovium-like Membrane to the Particulate Biomaterial in Murine Air-Pouch Model

Affiliations
  • 1Department of Orthopaedic Surgery, Seoul Municipal Boramae Hospital, Seoul, Korea.

Abstract


OBJECTIVES
The purpose of this study was to evaluate and classify the synovium-like membrane as either inflammatory (foreign body-like) or immune (T-cells present), generated during the development of aseptic loosening in the patients with joint replacement arthroplasties.
METHODS
We used the murine air-pouch model, developed in BALB/c mouse, to make artificial synovial cavity and assessed the cytokine responses of the synovium-like membrane to the particulate biomaterials such at pure titanium, Ti6Al4V alloy, CoCr alloy and UHMWPE. Each biomaterial injected into air-pouch of 20 mice respectively. The mRNAs of various cytokines (IL-1 beta, IL-6, TNFalpha, IL-4, gammaIFN) and TCR Calpha chain were extracted from the synovium-like membranes and RT-PCR reactions were carried out using appropriate primers.
RESULTS
IL-1beta, IL-6 and TNFalpha showed an increased response to the all kinds of particulate biomaterials. IL-4, gammaIFN and TCR Calpha chain did not show any distinct responses compared to control group and various kinds of biomaterials failed to show any, significant differences from each other in cytosine responses.
CONCLUSION
T-cell or immune cytokine responses had not been found in this particle reaction and it seemed to be that the osteolysis in aseptic loosening would be a biologic reaction to the particulate biomaterials due to an inflammatory process.

Keyword

Biomaterial; Particle; Macrophage; T-cell; Cytokine

MeSH Terms

Alloys
Animals
Arthroplasty, Replacement
Biocompatible Materials
Cytokines
Cytosine
Humans
Interleukin-4
Interleukin-6
Joints
Macrophages
Membranes*
Mice
Osteolysis
RNA, Messenger
T-Lymphocytes
Titanium
Tumor Necrosis Factor-alpha
Alloys
Biocompatible Materials
Cytokines
Cytosine
Interleukin-4
Interleukin-6
RNA, Messenger
Titanium
Tumor Necrosis Factor-alpha
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