J Korean Assoc Oral Maxillofac Surg.  2008 Aug;34(4):419-427.

The effects of beta-TCP/rhBMP-2 on bone formation in osteoblast-like cells induced from bone marrow-derived mesenchymal stem cells

• Choi YS
• Hwang KG
• Lee JS
• Park CJ
• Shim KS

Abstract

The present study aimed to investigate the osteogenic potentials of differentiated osteoblast-like cells (DOCs) induced from bone marrow-derived mesenchymal stem cells (MSCs) on beta-tricalcium phosphate (beta-TCP) with recombinant human bone morphogenetic protein (rhBMP-2) in vitro. Osteoblast differentiation was induced in confluent cultures by adding 100 nM dexamethasone, 10 mM beta-glycerophosphate, 50 mM L-ascorbic acid. The Alizarin red S staining and reverse transcriptase-polymerase chain reaction (RT-PCR) were perfomed to examine the mRNA expression of alkaline phosphatase (ALP), bone sialoprotein (BSP), osteocalcin (OCN), receptor activator for nuclear factor kappaB ligand (RANKL), runt-related transcription factor 2 (RUNX2), collagen-I (COL-I). There were no significant differences in the osteogenic potentials of DOCs induced from MSCs on beta-TCP(+/-). According to the incubation period, there were significant increasing of Alizadin red S staining in the induction 3 weeks. The mRNA expression of ALP, RUNX2, and RANKL were higher in DOCs/beta-TCP(-) than DOCs/beta-TCP(+). According to rhBMP-2 concentrations, the mRNA expression of BSP was significantly increased in DOCs/beta-TCP(+) compared to that of DOCs/beta-TCP(-) on rhBMP 10 ng/ml. Our study presented the beta-TCP will have the possibility that calcium phosphate directly affect the osteoblastic differentiation of the bone marrowderived MSCs.

Keyword

mesenchymal stem cell; rhBMP -2; beta-TCP; differentiated osteoblast-like cell

MeSH Terms

Alkaline Phosphatase
Anthraquinones
Ascorbic Acid
Bone Morphogenetic Proteins
Calcium
Calcium Phosphates
Dexamethasone
Durapatite
Glycerophosphates
Humans
Integrin-Binding Sialoprotein
Mesenchymal Stromal Cells
Osteoblasts
Osteocalcin
Osteogenesis
RNA, Messenger
Transcription Factors
Alkaline Phosphatase
Anthraquinones
Ascorbic Acid
Bone Morphogenetic Proteins
Calcium
Calcium Phosphates
Dexamethasone
Durapatite
Glycerophosphates
Integrin-Binding Sialoprotein
Osteocalcin
RNA, Messenger
Transcription Factors