J Korean Assoc Oral Maxillofac Surg.  2008 Feb;34(1):49-58.

The BMPs expression and histomorphometric study of beta-TCP /rhBMP-2 Grafting on the rabbit cranial bone defects

• Lim BS
• Jeon JY
• Park CJ
• Im JJ
• Hwang KG
• Shim KS

Abstract

OBJECTIVE
The Purpose of the study was to investigate the bone morphogenic protein expression of rhBMP-2(recombinant human bone morphogenic protein-2) as singnaling molecule and beta-TCP(Tricalcium phosphate) as a bone substitute and carrier medium of rhBMP-2.
MATERIALS AND METHODS
16 rabbits divided into 2 group of each 8 rabbit. Two standardized bone defect, round bilateral defect was made in the cranium of the 8 rabbit of first group, and was grafted with 150~500micrometer diameter beta-TCP 0.25g in one side, which was soaked with rhBMP-2, and autogenous bone was grafted on another side as a positive control. Second group of 8 rabbit, only beta-TCP was grafted with same size and same manner. After 2, 4, 8, and 12 weeks, specimen was taken for microscopic immunohiostochemical and histomorphometric analysis. RESULT: Grafting beta-TCP with rhBMP show the early formation of the bone regenerative factor (BMP-4) and more quantity of new bone formation than only use of beta-TCP (8,12 week), even show less new bone formation than autogenous bone.
CONCLUSION
The experimental study result that beta-TCP graft combination with rhBMP-2 as a delivery system is an effective with osteoinductive capacity and biodegradable properties, so that provide clinical availibility of composite use in reconstruction of bony defect.

Keyword

Beta-Tricalcium phosphate; bone graft; Bone Morphogenic Protein; bone substitute; histomorphometry; Immunohistochemistry

MeSH Terms

Bone Substitutes
Calcium Phosphates
Humans
Immunohistochemistry
Osteogenesis
Rabbits
Skull
Transplants
Bone Substitutes
Calcium Phosphates