J Korean Assoc Oral Maxillofac Surg.  2010 Jun;36(3):161-171. 10.5125/jkaoms.2010.36.3.161.

Morphometric analysis on bone formation effect of beta-TCP and rhBMP-2 in rabbit mandible

• Kim KN
• Yang JE
• Jang JW
• Sasikala B
• Beng W
• Kim IK

• KMID: 2189943
• DOI: http://doi.org/10.5125/jkaoms.2010.36.3.161

Abstract

INTRODUCTION
This study was to assess the effectiveness of new bone formation and regeneration by using a rhBMP-2 and beta-TCP as a carrier in rabbits' mandible.
MATERIALS AND METHODS
The mandibles of 36 rabbits were exposed and cortical bone was penetrated for this study. The experimental subjects were divided into 3 groups each 12 rabbits; control group, experimental group 1, and experimental group 2. Control group had the defect itself without any treatment, in the experimental group 1, beta-TCP only was grafted, and in the experimental group 2, rhBMP-2 soaked in beta-TCP was grafted. The rabbits were sacrificed after 1, 2, 3, 4, 6, and 8weeks, and new bone formation area was examined and measured for bone quantitative and qualitative analysis with light, fluorescent and polarized microscopy.
RESULTS
In the experimental group 1, new bone formation from the adjacent host bone was made by osteoconduction, and in the experimental group 2, direct new bone formation by osteoinduction of rhBMP-2 as well as new bone formation by osteoconduction of beta-TCP were observed.
CONCLUSION
rhBMP-2 of experimental group 2 is very effective in the bone formation in early 2weeks and bone remodelling from 3weeks.

Keyword

Bone morphogenetic protein; Tricalcium Phosphate (TCP); Osteoconduction; Osteoinduction

MeSH Terms

Bone Morphogenetic Proteins
Bone Regeneration
Calcium Phosphates
Light
Mandible
Microscopy
Osteogenesis
Rabbits
Regeneration
Transplants
Bone Morphogenetic Proteins
Calcium Phosphates

Figure

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  Fig. 1. Circular defect form in rabbit's mandible.

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  Fig. 2. Photomicrographs of the histological section seen by light microscopy.(original magnification x10) (TCP: β-tricalcium phosphate)

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  Fig. 3. Photomicrographs of decalcified specimen.(H&E staining) A. Experimental group 1, 2 weeks.(original magnification x200) B. Experimental group 2, 2 weeks.(original magnification x200) (TCP: β-tricalcium phosphate)

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  Fig. 4. Photomicrographs of decalcified specimen.(H&E staining) A. Experimental group 1, 6 weeks.(original magnification x200) B. Experimental group 2, 6 weeks.(original magnification x200) (TCP: β-tricalcium phosphate)

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  Fig. 5. Quantitative analysis of bone formation at TCP/rhBMP-2 grafts. (H&E staining, original magnification x100, 4 weeks) Boundary of new bone formation were marked and calculated with Image Pro Plus 4.0.(A1-A10) (TCP: β-tricalcium phosphate, rhBMP-2: recombinant human bone morphogenetic protein-2)

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  Fig. 6. Quantitative analysis of bone formation at control group (defect only), TCP grafts only and TCP/rhBMP-2 grafts. (H&E staining, original magnification x100) (TCP: β-tricalcium phosphate, rhBMP-2: recombinant human bone morphogenetic protein-2)

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  Fig. 7. Quantitative analysis of bone formation at control group (defect only), β-TCP grafts only and β-TCP/rhBMP-2 grafts. (TCP: β-tricalcium phosphate, rhBMP-2: recombinant human bone morphogenetic protein-2)

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  Fig. 8. Photomicrographs of undecalcified specimen.(original magnification x100) A. Experimental group 2, 8 weeks. (Villanueva bone stain) B. Experimental group 2, 8 weeks.(fluorescence light) (TCP: β-tricalcium phosphate)

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  Fig. 9. Comparison of Villanueva bone stain and fluorescence light microscopic photographs of undecalcified specimen at control group (defect only), TCP grafts only and TCP/rhBMP-2 grafts. (TCP: β-tricalcium phosphate, rhBMP-2: recombinant human bone morphogenetic protein-2)

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  Fig. 10. Photomicrographs of undecalcified specimen.(original magnification x100) A. Experimental group 2, 8 weeks.(Villanueva bone stain) B. Experimental group 2, 8 weeks.(polarizing light) (TCP: β-tricalcium phosphate)

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  Fig. 11. Comparison of Villanueva bone stain and polarizing light microscopic photographs of undecalcified specimen at control group (defect only), TCP grafts only and TCP/rhBMP-2 grafts. (TCP: β-tricalcium phosphate, rhBMP-2: recombinant human bone morphogenetic protein-2)

Reference

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