The Effect of PRF and PRP for New Bone Formation of beta-TCP in Skull of White Rabbit

Park, Jeong Kyun; Joo, Hyun Jung; Lee, Ei Seok; Jang, Hyon Seok; Lim, Jae Seok; Kwon, Jong Jin

Abstract

PURPOSE
Addition of platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) to grafting material has become widely accepted additively for bone regeneration because it can raise high expectations on it's clinical potential. The aim of this study was to evaluate the efficacy of PRP and PRF on early bone regeneration of rabbits when used in combination with beta tricalcium phosphate.
METHODS
In eight rabbits, the calvarium was exposed and the two marrows were penetrated. After then these artificial bone defects were augmented with beta-TCP or beta-TCP with PRP or beta-TCP with PRF and covered. The animals were sacrificed after four and eight weeks. Histologic findings were observed under the light-microscope and histomorphometric analysis was performed by measuring calcified area of new bone formation within the CSD.
RESULTS
They demonstrated that new bone formation tended to be produced along the outline of graft materials. More amounts of newly bone was regenerated in beta-TCP only and in combination ofbeta-TCP with PRF and it was statistically significant. In contrast, there was no significant difference between nothing apply and beta-TCP with PRP groups in the relative amounts of newly mineralized bone.
CONCLUSION
Within the limitation of this study, it can be concluded that PRF in combination with beta-TCP showed a positive effect on bone regeneration and statistically it was significant.