J Korean Acad Rehabil Med.  2011 Feb;35(1):8-13.

Myogenic Differentiation of Human Adipose-Derived Stem Cells

• Park YG
• Baek AM
• Do BR
• Choi JH
• Kim SD

Abstract

OBJECTIVE
Cell therapy has been extensively studied as a gene complementation approach in muscular dystrophy including Duchenne muscular dystrophy (DMD), and adipose tissue has recently been identified as a uniquely abundant and adequately accessible source of pluripotent cells. In the present work, we investigated myogenic potentials of adipose-derived stem cells (ADSCs) depending on culture media and isolation with using surface markers. METHOD: Human ADSCs were obtained by liposuction and cultured in two different media; control and myogenic media. In addition we attempted to isolate ADSCs by utilizing surface markers: CD45 and CD133. The following observations were made to evaluate myogenic differentiation as the expression of myogenic regulatory factors (MyoD, Myf-5 and Myf-6) and desmin by RT-PCR and immunoflurescence study.
RESULTS
Conversion of ADSCs to myogenic phenotype was observed by indirect immunoflurescence study of MyoD and Myf-5 in regardless of media type and isolation method. In addition mRNA of MyoD and Myf-5 were positive in both culture media, and there were no differences of MyoD and Myf-5 responses between CD45- and CD45-CD133-ADSCs. However, secondary myogenic regulatory factor (Myf-6) was not expressed constantly, and desmin were negative in all cultural condition.
CONCLUSION
Our findings suggest that human ADSCs might have myogenic potentials. However, further studies are needed to express the secondary myogenic regulatory factors and proteins in myoblasts.

Keyword

Muscular dystrophy; Stem cell; Differentiation; Myogenic regulatory factor; Desmin

MeSH Terms

Adipose Tissue
Complement System Proteins
Culture Media
Desmin
Genes, vif
Humans
Lipectomy
Muscular Dystrophies
Muscular Dystrophy, Duchenne
Myoblasts
Myogenic Regulatory Factors
Phenotype
Proteins
RNA, Messenger
Stem Cells
Tissue Therapy
Complement System Proteins
Culture Media
Desmin
Myogenic Regulatory Factors
Proteins
RNA, Messenger