J Korean Burn Soc.
2011 Dec;14(2):93-96.
The Rapid Establishment of Human Clonal Adipose Derived Stem Cell (hADSC) Lines with Aspirated Adipose Tissue
- Affiliations
-
- 1Department of Biomedical Science, CHA University, Seongnam, Korea. suman@cha.ac.kr
- 2Department of Obstetrics and Gynecology, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Korea.
- 3Department of Applied Bioscience, CHA University, Seongnam, Korea.
Abstract
- PURPOSE
This study aims to establish a new strategy that provides for the rapid establishment of human clonal adipose derived stem cell (hADSC) lines with aspirated adipose tissue and to characterize newly generated hMSC lines for their cell phenotype, differentiation potential, lineage-specific gene expression.
METHODS
Human adipose tissue-derived stem cells (hADSCs) were isolated from subcutaneous adipose tissue based on standard protocols. After incubation for 2 h, only the cell culture supernatant was transferred to a new dish. This process was repeated several times with 30 h incubations.
RESULTS
We confirmed the difference in growth rate, however, differences were not seen in the differentiation capabilities and stemness of the each cell lines.
CONCLUSION
It is necessary to establish cell lines via single cell level for application to disease specific tissue engineering.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Adipose-derived stem cells: characterization and clinical application
- Comparison with human amniotic membrane- and adipose tissue-derived mesenchymal stem cells
- Transplantation of Human Adipose-derived Stromal Cells Promotes Functional Recovery of Rat Spinal Cord Injury
- Extracellular Vesicles from Adipose-Derived Stem Cells Promote Diabetic Wound Healing via the PI3K-AKT-mTOR-HIF-1a Signaling Pathway
- Adipose Tissue-Derived Stem Cells for Myocardial Regeneration
