Anat Cell Biol.  2013 Jun;46(2):113-121. 10.5115/acb.2013.46.2.113.

Expression of surface markers and myogenic potential of rat bone marrow- and adipose-derived stem cells: a comparative study

Affiliations
  • 1Cellular and Molecular Research Center, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. bayati-v@ajums.ac.ir
  • 2Department of Anatomical Science, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
  • 3Department of Anatomy and Cell Biology, Gilan University of Medical Sciences, Rasht, Iran.
  • 4Department of Anatomical Sciences, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.

Abstract

In recent years, examination and comparison of the biological characteristics of bone marrow- and adipose-derived mesenchymal stem cells (MSCs) from various perspectives have come into the focus of stem cell research, as these cells should be well characterized in order to utilize them in future cellular therapies. Therefore, in the present study, surface protein markers and the skeletal myogenic differentiation potential of rat bone marrow- and adipose-derived MSCs were examined. The expression of CD44, CD45, CD73, and CD90 on bone marrow- and adipose-derived MSCs was characterized using flow cytometry. Subsequently, the stem cells were differentiated into myogenic lineages, and the expression of the skeletal myogenic markers MyoD1, Myog, and Myh2 was studied in cells using real time polymerase chain reaction and immunofluorescence. Our results reveal that the pattern of CD marker expression differs between these 2 types of MSCs to some extent, whereas no significant difference was observed with respect to their myogenic differentiation potential. Therefore, we concluded that despite the differences observed in the biological features of these 2 types of MSCs, their myogenic potential appears to be similar, and that adipose-derived stem cells may be useful in skeletal muscle tissue engineering, due to their easy isolation and capacity for rapid expansion in a short time span.

Keyword

Surface antigens; Myogenic potential; Bone marrow-derived mesenchymal stem cells; Adipose-derived stem cells; Rat

MeSH Terms

Animals
Antigens, Surface
Flow Cytometry
Fluorescent Antibody Technique
Mesenchymal Stromal Cells
Muscle, Skeletal
Population Characteristics
Rats
Real-Time Polymerase Chain Reaction
Stem Cell Research
Stem Cells
Tissue Engineering
Antigens, Surface
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