Clin Should Elbow.  2012 Dec;15(2):65-72.

Differential Potential of Stem Cells Following Their Origin: Subacromial Bursa, Bone Marrow, Umbilical Cord Blood

• Sim SW
• Moon YL
• Kang JH

Abstract

PURPOSE
To evaluate the differentiation potential of stem cells and their immunophenotype from 3 different sources.
METHODS
Our study involved three stem cell sources-subacromial bursal tissue, bone marrow, and umbilical cord blood. We obtained the subacromial bursal tissue and bone marrow from the patients undergoing shoulder surgery. After collecting the sample, we applied specific induction media for neurogenic, adipogenic and osteogenic differentiation. Also, flow-cytometry analysis was done to reveal the cell surface antigens.
RESULTS
We obtained 100% (8 cases) neural and adipogenic differentiation, but 62.5% (5 of 8 cases) osseous differentiation among the subacromial bursal tissue group. Bone marrow derived cells showed 100% neural (6 cases) and adipogenic (5 cases) differentiation, but 80% (4 of 5 cases) osseous differentiation. Umbilical cord blood derived cells revealed 97% (65 of 67 cases) neural, 53.7% (29 of 54 cases) adipogenic and 68.4% (39 of 57 cases) osseous differentiation. Immunophenotype analysis revealed that surface markers of bone marrow, subacromial bursal cell and umbilical cord blood derived mesenchymal stem cells are different from each other.
CONCLUSIONS
Mesenchymal stem cells are potential agents in regenerative medicine and are characterized by expression of surface markers and by their differentiation potential. Our study with stem cells from subacromial bursal tissue, bone marrow and umbilical cord discovered that each stem cell has unique differentiation potential and function based on its origin. Various stem cells show multi-lineage differentiations in vitro which can be correlated to in vivo conditions.

MeSH Terms

Bone and Bones
Bone Marrow
Durapatite
Fetal Blood
Humans
Mesenchymal Stromal Cells
Regenerative Medicine
Shoulder
Stem Cells
Umbilical Cord
Durapatite