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High efficiency of homemade culture medium supplemented with GDF9-β in human oocytes for rescue in vitro maturation

Mohsenzadeh M, Khalili MA, Anbari F, Vatanparast M

Objective: Optimizing culture media for the incubation of immature oocytes is a vital strategy to increase the oocyte maturation rate during in vitro maturation (IVM) programs. This study evaluated the...
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Comparison of the Infant and Adult Adipose-Derived Mesenchymal Stem Cells in Proliferation, Senescence, Antioxidative Ability and Differentiation Potential

Wu SH, Yu JH, Liao YT, Liu KH, Chiang ER, Chang MC, Wang Jp

BACKGROUND: Infant adipose-derived mesenchymal stem cells (ADSCs) collected from excised polydactyly fat tissue, which was surgical waste, could be cultured and expanded in vitro in this study. In addition, the...
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Reduced Osteogenic Differentiation Potential In Vivo in Acute Myeloid Leukaemia Patients Correlates with Decreased BMP4 Expression in Mesenchymal Stromal Cells

Azevedo PL, Dias RB, Nogueira LP, Maradei S, Bigni R, Aragao JSR, Abdelhay E, Binato R

The osteogenic differentiation potential of mesenchymal stromal cells (hMSCs) is an essential process for the haematopoiesis and the maintenance of haematopoietic stem cells (HSCs). Therefore, the aim of this work...
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Evaluation of the Potential Effects of Retinol and Alginate/Gelatin-Based Scaffolds on Differentiation Capacity of Mouse Mesenchymal Stem Cells (MSCs) into Retinal Cells

Haghighat M, Iranbakhsh A, Baharara J, Ebadi M, Sotoodehnejadnematalahia F

Background and Objectives: Retinal stem cells (RSCs) resided in ciliary epithelium have shown to possess a high ca-pacity to self-renew and differentiate into retinal cells. RSCs could be induced to...
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The Differentiation of Pluripotent Stem Cells towards Endothelial Progenitor Cells – Potential Application in Pulmonary Arterial Hypertension

Qin K, Lei J, Yang J

Background and Objectives: Endothelial progenitor cells (EPCs) and endothelial cells (ECs) have been applied in the clinic to treat pulmonary arterial hypertension (PAH), a disease characterized by disordered pulmonary vasculature....
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Application of Bone Marrow Mesenchymal Stem Cells Effectively Eliminates Endotoxemia to Protect Rat from Acute Liver Failure Induced by Thioacetamide

Jiang T, Xia G, Yang B, Zhang Hw, Yin Ys, Tang Cw, Yang Jh

BACKGROUND: Endotoxemia is related to worse clinical outcomes in acute liver failure (ALF), but its management remains unsatisfactory. In this study, we aimed to assess whether the application of bone...
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Colorectal adenocarcinoma with enteroblastic differentiation: diagnostic challenges of a rare case encountered in clinical practice

Abada E, Anaya IC, Abada O, Lebbos A, Beydoun R

Colorectal adenocarcinoma with enteroblastic differentiation (CAED) is a rare subtype of colonic adenocarcinoma characterized by increased α-fetoprotein (AFP) production and the expression of at least one enteroblastic marker including AFP,...
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Multidimensional Biomarker Analysis Including Mitochondrial Stress Indicators for Nonalcoholic Fatty Liver Disease

Chang E, Chang JS, Kong ID, Baik SK, Kim MY, Park KS

Nonalcoholic fatty liver disease (NAFLD) is accompanied by a complex and multifactorial pathogenesis with sequential progressions from inflammation to fibrosis and then to cancer. This heterogeneity interferes with the development...
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A Simple Method for Generating Cerebral Organoids from Human Pluripotent Stem Cells

Hong YJ, Lee Sb, Choi J, Yoon SH, Do JT

Background and Objectives: In recent years, brain organoid technologies have been the most innovative advance in neural differentiation research. In line with this, we optimized a method to establish cerebral organoids...
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Direct Conversion to Achieve Glial Cell Fates: Oligodendrocytes and Schwann Cells

Yun W, Kim YJ, Lee G

Glia have been known for its pivotal roles in physiological and pathological conditions in the nervous system. To study glial biology, multiple approaches have been applied to utilize glial cells...
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Secretome of Stem Cells: Roles of Extracellular Vesicles in Diseases, Stemness, Differentiation, and Reprogramming

Kim HJ, Kim G, Lee J, Lee Y, Kim JH

Increasing evidence suggests that stem cells or stem cell-derived cells may contribute to tissue repair, not only by replacing lost tissue but also by delivering complex sets of secretory molecules,...
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The Role of Histone Acetylation in Mesenchymal Stem Cell Differentiation

Jang S, Hwang J, Jeong HS

The mechanism and action concerning epigenetic modifications, especially that of histone modifications, are not fully understood. However, it is clear that histone modifications play an essential role in several biological...
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Nicotinamide phosphoribosyltransferase regulates the cell differentiation and mineralization in cultured odontoblasts

Kang KR, Kim JS, Seo JY, Lim H, Kim TH, Yu SK, Kim HJ, Kim CS, Chun HS, Park JC, Kim DK

The aim of the present study was to investigate the physiological role of nicotinamide phosphoribosyltransferase (NAMPT) associated with odontogenic differentiation during tooth development in mice. Mouse dental papilla cell-23 (MDPC-23)...
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Catalpol Inhibits Tregs-to-Th17 Cell Transdifferentiation by Up-Regulating Let-7g-5p to Reduce STAT3 Protein Levels

Di Y, Zhang M, Chen Y, Sun R, Shen M, Tian F, Yang P, Qian F, Zhou L

Purpose: Rheumatoid arthritis (RA) is a chronic systemic autoimmune disease, and Th17 cells are key factors in the pathogenesis of human inflammatory conditions, such as RA. Catalpol (CAT), a component...
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Biocompatibility and Bioactivity of Four Different Root Canal Sealers in Osteoblastic Cell Line MC3T3-El

Jun NR, Lee SK, Lee SI

Background: Endodontic sealers or their toxic components may become inflamed and lead to delayed wound healing when in direct contact with periapical tissues over an extended period. Moreover, an overfilled...
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MiR-148a-3p Regulates the Invasion and Odontoblastic Differentiation of Human Dental Pulp Stem Cells via the Wnt1/β-Catenin Pathway

Li Q, Huang L

Background and Objectives: MiR-148a-3p has been reported to regulate the differentiation of marrow stromal cell osteoblast. In this study, whether miR-148a-3p regulated the odontoblastic differentiation of human dental pulp...
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Nigella Sativa-Coated Hydroxyapatite Scaffolds: Synergetic Cues to Stimulate Myoblasts Differentiation and Offset Infections

Amna T, A. Alghamdi AA, Shang K, Hassan MS

BACKGROUND: At present osteoporosis has come into view as a major health concern. Skeletal diseases typified by weak and fragile bones have imposed threats of fissure. Hydroxyapatite (HAP) is known...
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Alteration of TGFB1, GDF9, and BMPR2 gene expression in preantral follicles of an estradiol valerate-induced polycystic ovary mouse model can lead to anovulation, polycystic morphology, obesity, and absence of hyperandrogenism

Asghari R, Shokri-Asl V, Rezaei H, Tavallaie M, Khafaei M, Abdolmaleki A, Majdi Seghinsara A

Objective: In humans, polycystic ovary syndrome (PCOS) is an androgen-dependent ovarian disorder. Aberrant gene expression in folliculogenesis can arrest the transition of preantral to antral follicles, leading to PCOS. We...
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Potential Role of Cytosolic RNA Sensor MDA5 as an Inhibitor for Keratinocyte Differentiation in the Pathogenesis of Psoriasis

Hong DK, Choi MR, Hwang YL, Lee JK, Lee Y, Seo YJ, Kim S, Lee YH, Kim CD, Lee JH

Background: Psoriasis is a chronic inflammatory skin disease. The etiology of psoriasis is not fully understood, but the genetic background is considered to be the most important factor. To date,...
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Rapid Differentiation of Human Embryonic Stem Cells into Testosterone-Producing Leydig Cell-Like Cells In vitro

Shin EY, Park S, Choi WY, Lee DR

BACKGROUND: Leydig cells (LCs) are testicular somatic cells that are the major producers of testosterone in males. Testosterone is essential for male physiology and reproduction. Reduced testosterone levels lead to hypogonadism...
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