J Korean Surg Soc.
2006 Jul;71(1):31-38.
Effect of Antibiotics on the Survival of Human Hepatocellular Carcinoma Cells under Hypoxic Conditions
- Affiliations
-
- 1Department of Biochemistry, College of Medicine, The Catholic University of Daegu, Korea. leejw@cu.ac.kr
- 2Department of Surgery, College of Medicine, The Catholic University of Daegu, Korea.
- 3Department of Laboratory Medicine, College of Medicine, The Catholic University of Daegu, Korea.
- 4Department of Pathology, College of Medicine, The Catholic University of Daegu, Korea.
- 5Department of Surgery, Kwang-Hae Hospital, Korea.
- 6The Wistar Institute, Korea.
- 7Department of Surgery, College of Medicine, Kyungpook National University, Daegu, Korea.
Abstract
-
PURPOSE: Antibiotics that kill or suppress the growth of bacteria also affect tumors directly or indirectly. The authors aimed to show whether some antibiotics can improve cancer cell survival under hypoxic conditions, and how the antibiotics improve the cells under hypoxic conditions.
METHODS
Human hepatocellular carcinoma cells (HepG2) were grown at 1% oxygen concentration. Cell numbers, glucose concentrations and lactic acid concentrations in the medium were measured at different incubation times, in the absence or presence of aminoglycosides, tetracyclines, quinolones, penicillins, cephalosporins, sulfonamides, or chloramphenicols. DNA fragmentation assay was performed to study the mechanism how some antibiotics improve the cell survival under hypoxic conditions.
RESULTS
Of the antibiotics tested, only aminoglycosides, tetracyclines, quinolones and the chloramphenicol improved cell survival under hypoxic conditions. Geneticin (G418), an aminoglycoside chosen as an example, improved cell survival even if glucose in the medium was completely consumed. At the same time, the appearance of DNA ladder was delayed in the presence of geneticin, which was also the same for the other antibiotics that improved cell survival under hypoxic conditions.
CONCLUSION
Some antibiotics improved hepatocellular carcinoma cells under ischemic conditions by inhibiting apoptosis. The results implies that the antibiotics might adversely affect solid tumors, by improving cancer cell growth where hypoxic or ischemic conditions occur in the core region. Therefore, we might be cautious in choosing antibiotics for cancer patients with solid tumors, especially when the patients should be treated with antibiotics for a long time.
Keyword
MeSH Terms
-
Aminoglycosides
Anoxia
Anti-Bacterial Agents*
Apoptosis
Bacteria
Carcinoma, Hepatocellular*
Cell Count
Cell Survival
Cephalosporins
Chloramphenicol
DNA
DNA Fragmentation
Glucose
Humans*
Lactic Acid
Oxygen
Penicillins
Quinolones
Sulfonamides
Tetracyclines
Aminoglycosides
Anti-Bacterial Agents
Cephalosporins
Chloramphenicol
DNA
Glucose
Lactic Acid
Oxygen
Penicillins
Quinolones
Sulfonamides
Tetracyclines
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Effect of antitumor agents on the survival of human hepatocellular carcinoma cells under hypoxic conditions
- Effect of Combination of Anticancer Agents and Nitroimidazoles on the Survival of Human Hepatocellular Carcinoma Cells under Hypoxic Conditions
- The Effect of Doxycycline on the Survival of the Cells under Hypoxic Condition
- Studies on the Mechanism of Hypoxic Increase of VEGF Expression in the Hep3B Human Hepatoma Cells
- Kupffer Cells in Hepatocellular Carcinoma
