Korean J Anat.
2001 Oct;34(5):517-524.
Developmental mRNA Expression of cdk5 and its Putative Regulators, p67 and p35, in Rat Brain
- Affiliations
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- 1Department of Anatomy and Institute of Human Genetics, Korea University, College of Medicine, Korea. kimhyun@kuccnx.korea.ac.kr
- 2Department of Anatomy, College of Medicine, Ewha Womans University, Korea.
Abstract
- Cyclin-dependent kinase 5 (cdk5) is essential for brain development and p35 and p67 are the regulatory molecules for cdk5. In this study, we have investigated the expression of cdk5, p35, and p67 mRNAs in the developing rat brain with in situ hybridization histochemistry. The expression of cdk5 mRNA was already observed in embryonic day 12 (E12), start point of neurogenesis in rat brain, throughout the brain and gradually increased until postnatal day 3 (P3). At this period, strong expression of cdk5 mRNA was observed in the cerebral cortex, hippocampus, dentate gyrus, thalamus, hypothalamus, and inferior colliculus. High level of cdk5 expression was maintained in the postnatal rat brain and prominent expression was observed in the hippocampus, dentate gyrus, cerebellum, and choroid plexus of adult rat brain. Strong expression of p35 mRNA was observed between E16 and E20 in the cerebral cortex, hippocampus, dentate gyrus, thalamus, hypothalamus, and inferior colliculus as like as cdk5. After birth, the expression of p35 mRNA was gradually decreased and significant differences in the expression of cdk5 and p35 were observed in the thalamus, hypothalamus, midbrain, and cerebellum. In the embryonic period, the expression pattern of p67 was very similar with that of p35 but expression level was lower than p35. After birth, strong expression of p67 was observed in the areas where the expression of cdk5 was high. From these results, it is suspected that p35 may function in neuronal migration, and p67 in differentiation and maturation, as a major regulator for cdk5 in developing rat brain.
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