Korean J Physiol Pharmacol.  2011 Oct;15(5):267-272. 10.4196/kjpp.2011.15.5.267.

Characteristics of 5-Hydroxytryptamine Receptors Involved in Contraction of Feline Ileal Longitudinal Smooth Muscle

• Wang Y
• Park SY
• Oh KH
• Min Y
• Lee YJ
• Lee SY
• Sohn UD

• KMID: 2071728
• DOI: http://doi.org/10.4196/kjpp.2011.15.5.267

Abstract

A number of studies have demonstrated that 5-hydroxytryptamine (5-HT) can induce muscle contraction or relaxation response and enhance secretion in the gastrointestinal tract via a multiplicity of 5-HT receptor subtypes. In the present study, we investigated the pharmacological characterization of the 5-HT-induced contractile response in longitudinal smooth muscle isolated from the feline ileum. Addition of 5-HT into muscle chambers enhanced the basal tone and spontaneous activity in a concentration-dependent manner. The neurotoxin tetrodotoxin did not alter the 5-HT-induced contraction of the longitudinal muscles. Neither atropine nor guanethidine affected the contraction. The 5-HT agonists, 5-methylserotonin hydrochloride and mosapride, also evoked concentration-dependent contractions. The 5-HT-induced contraction was enhanced by the 5HT2 receptor antagonist ketanserin and the 5-HT3 receptor antagonist ondansetron but was inhibited by the 5-HT1 receptor antagonist methysergide and 5-HT4 receptor antagonist GR113808. These results indicate that 5-HT1 and 5-HT4 receptors may mediate the contraction of the 5-HT-induced response and 5-HT2 and 5-HT3 receptors may mediate 5-HT-induced relaxation in feline ileal longitudinal smooth muscles.

Keyword

Ileum; Longitudinal smooth muscle; 5-HT; Contraction; Relaxation

MeSH Terms

Atropine
Benzamides
Contracts
Gastrointestinal Tract
Guanethidine
Ileum
Indoles
Ketanserin
Methysergide
Morpholines
Muscle Contraction
Muscle, Smooth
Muscles
Ondansetron
Receptors, Serotonin
Receptors, Serotonin, 5-HT1
Receptors, Serotonin, 5-HT3
Receptors, Serotonin, 5-HT4
Relaxation
Serotonin
Serotonin Receptor Agonists
Sulfonamides
Tetrodotoxin
Atropine
Benzamides
Guanethidine
Indoles
Ketanserin
Methysergide
Morpholines
Ondansetron
Receptors, Serotonin
Receptors, Serotonin, 5-HT1
Receptors, Serotonin, 5-HT3
Receptors, Serotonin, 5-HT4
Serotonin
Serotonin Receptor Agonists
Sulfonamides
Tetrodotoxin

Figure

• 

  Fig. 1. Representative tracing showing the effect of 5-HT on the spontaneous contraction of feline longitudinal smooth muscle. 5-HT was added cumulatively (10–10∼10–6 M) with 5-min contact time for each concentration.

• 

  Fig. 2. Effect of atropine or guanethidine on 5-HT-induced contraction. Concentration-response curves to 5-HT in the absence (❍) and presence of 0.1 μM atropine (), (A) or 1 μM guanethidine (▪), (B). Preparations were incubated with atropine or guanethidine for 10 min before and during 5-HT treatment. Each point represents the mean±S.E.M. (n=6).

• 

  Fig. 3. Effect of TTX on 5-HT-induced contraction. Concentration-response curves to 5-HT in the absence (❍) and presence of 0.1 μM () and 1 μM (▪) TTX. Preparations were incubated with TTX for 10 min before and during 5-HT treatment. Each point represents the mean±S.E.M. (n=5).

• 

  Fig. 4. Concentration-response curves for 5-HT- (❍, n=11), 5-MOT-(, n=8) and mosapride- (▪, n=10) induced contraction. Each point represents the mean±S.E.M.

• 

  Fig. 5. Inhibitory effect of 5-HT receptor antagonists on 5-HT-induced contraction. The preparation was incubated with (A) methysergide (1 μM, n=5), (B) ketanserin (0.1 μM, n=8), (C) ondansetron (1 μM, n=8) or (D) GR113808 (10 nM, n=5) for 30 min before and during 5-HT treatment. Absence (❍) or presence () of antagonist. Each point represents the mean±S.E.M. ∗p<0.05 and ∗∗p<0.01 compared to the same concentration of 5-HT-induced contraction alone.

• 

  Fig. 6. Major roles of 5-HT receptors in isolated feline ileum longitudinal smooth muscle. 5-HT can stimulate 5-HT1 or 5-HT4 receptors resulting in contraction of smooth muscle. 5-HT can also stimulate 5-HT2 or 5-HT3 receptors resulting in relaxation of smooth muscle.

Reference

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