Korean J Physiol Pharmacol.  2010 Oct;14(5):311-316. 10.4196/kjpp.2010.14.5.311.

P2X and P2Y Receptors Mediate Contraction Induced by Electrical Field Stimulation in Feline Esophageal Smooth Muscle

• Cho YR
• Jang HS
• Kim W
• Park SY
• Sohn UD

• KMID: 2071699
• DOI: http://doi.org/10.4196/kjpp.2010.14.5.311

Abstract

It is well-known that electrical field stimulation (EFS)-induced contraction is mediated by a cholinergic mechanism and other neurotransmitters. NO, ATP, calcitonin gene-related peptide (CGRP), and substance P are released by EFS. To investigate the purinergic mechanism involved in the EFS-induced contraction, purinegic receptors antagonists were used. Suramine, a non-selective P2 receptor antagonist, reduced the contraction induced by EFS. NF023 (10(-7)~10(-4) M), a selective P2X antagonist, inhibited the contraction evoked by EFS. Reactive blue (10(-6)~10(-4) M), selective P2Y antagonist, also blocked the contraction in a dose-dependent manner. In addition, P2X agonist alpha,beta-methylene 5'-adenosine triphosphate (alphabetaMeATP, 10(-7)~10(-5) M) potentiated EFS-induced contraction in a dose-dependent manner. P2Y agonist adenosine 5'-[beta-thio]diphosphate trilithium salt (ADPbetaS, 10(-7)~10(-5) M) also potentiated EFS-induced contractions in a dose-dependent manner. Ecto-ATPase activator apyrase (5 and 10 U/ml) reduced EFS-induced contractions. Inversely, 6-N,N-diethyl-D-beta,gamma-dibromomethylene 5'-triphosphate triammonium (ARL 67156, 10(-4) M) increased EFS-induced contraction. These data suggest that endogenous ATP plays a role in EFS-induced contractions which are mediated through both P2X-receptors and P2Y-receptors stimulation in cat esophageal smooth muscle.

Keyword

Electrical field stimulation; Smooth muscle; G protein; P2 receptor; ATP; Calcium

MeSH Terms

Adenosine
Adenosine Triphosphatases
Adenosine Triphosphate
Animals
Apyrase
Calcitonin Gene-Related Peptide
Calcium
Cats
Contracts
GTP-Binding Proteins
Muscle, Smooth
Neurotransmitter Agents
Polyphosphates
Substance P
Suramin
Adenosine
Adenosine Triphosphatases
Adenosine Triphosphate
Apyrase
Calcitonin Gene-Related Peptide
Calcium
GTP-Binding Proteins
Neurotransmitter Agents
Polyphosphates
Substance P
Suramin

Figure

• 

  Fig. 1. Effect of the P2 receptor antagonist on the response of EFS-induced contraction. Suramine (a P2 receptor antagonist) inhibited EFS-induced contraction in a concentration-dependent manner, suggesting that P2 receptors participate in muscle contraction in response to EFS. Values are expressed as means± S.E.M. ∗p<0.05, ∗∗p<0.01 versus the control.

• 

  Fig. 2. Effect of the P2X receptor antagonist on the response of EFS-induced contraction. NF023 (a P2X receptor antagonist) inhibited EFS-induced contraction in a concentration-dependent manner, suggesting that P2X receptors participate in muscle contraction in response to EFS. Values are expressed as means± S.E.M. ∗∗p<0.01 versus the control.

• 

  Fig. 3. Effect of the P2X receptor agonist on the response of EFS-induced contraction. α,β-meATP, P2X receptor agonist, potentiated EFS-induced contractions, suggesting that EFS-induced contraction involves P2X receptors, mainly P2X1. Values are expressed as means±S.E.M. ∗p<0.05 versus the control.

• 

  Fig. 4. Effect of the P2Y receptor antagonist on the response of EFS-induced contraction. Reactive blue (a P2Y receptor antagonist) inhibited EFS-induced contraction in a concentration-dependent manner, suggesting that P2Y receptors participate in muscle contraction in response to EFS. Values are expressed as means± S.E.M. ∗p<0.05, ∗∗p<0.01 versus the control.

• 

  Fig. 5. Effect of the P2Y receptor agonist on the response of EFS-induced contraction. ADPβS, a P2Y receptor agonist, potentiated EFS-induced contractions, suggesting EFS-induced contraction involves P2Y receptors, mainly P2Y1. Values are expressed as means±S.E.M. ∗p<0.05 versus the control.

• 

  Fig. 6. Effect of apyrase on the response of EFS-induced contraction. Apyrase (an ecto-ATPase activator, n=4) inhibited EFS-induced contractions, suggesting endogenous ATP is released upon EFS. Values are expressed as means±S.E.M. ∗p<0.05 versus the control.

• 

  Fig. 7. Effect of ARL67156 on the response of EFS-induced contraction. ARL 67156 (an ecto-ATPase inhibitor, n=4) increased EFS-induced contractions, suggesting endogenous ATP is released upon EFS. Values are expressed as means±S.E.M. ∗p<0.05 versus the control.

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