Biomol Ther.  2013 Sep;21(5):349-357.

6'-O-Galloylpaeoniflorin Protects Human Keratinocytes Against Oxidative Stress-Induced Cell Damage

• Yao CW
• Piao MJ
• Kim KC
• Zheng J
• Cha JW
• Hyun JW

Abstract

6'-O-galloylpaeoniflorin (GPF) is a galloylated derivate of paeoniflorin and a key chemical constituent of the peony root, a perennial flowering plant that is widely used as an herbal medicine in East Asia. This study is the first investigation of the cytoprotective effects of GPF against hydrogen peroxide (H2O2)-induced cell injury and death in human HaCaT keratinocytes. GPF demonstrated a significant scavenging capacity against the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical, H2O2-generated intracellular reactive oxygen species (ROS), the superoxide anion radical (O2-), and the hydroxyl radical (*OH). GPF also safeguarded HaCaT keratinocytes against H2O2-provoked apoptotic cell death and attenuated oxidative macromolecular damage to DNA, lipids, and proteins. The compound exerted its cytoprotective actions in keratinocytes at least in part by decreasing the number of DNA strand breaks, the levels of 8-isoprostane (a stable end-product of lipid peroxidation), and the formation of carbonylated protein species. Taken together, these results indicate that GPF may be developed as a cytoprotector against ROS-mediated oxidative stress.

Keyword

6'-O-Galloylpaeoniflorin; HaCaT keratinocyte; Hydrogen peroxide; Oxidative stress; Reactive oxygen species

MeSH Terms

Cell Death
DNA
Far East
Flowers
Herbal Medicine
Humans*
Hydrogen Peroxide
Hydroxyl Radical
Keratinocytes*
Oxidative Stress
Paeonia
Plants
Reactive Oxygen Species
Superoxides
DNA
Hydrogen Peroxide
Hydroxyl Radical
Reactive Oxygen Species
Superoxides