Korean J Otorhinolaryngol-Head Neck Surg.  2009 Nov;52(11):885-892. 10.3342/kjorl-hns.2009.52.11.885.

Effects of Nasal Instillation of Staphylococcal Enterotoxin B on Allergic Inflammation in Mice

• Kim YM
• Kim EH
• Park CH
• Yoon YH
• Kim JM
• Rha KS

• KMID: 1978392
• DOI: http://doi.org/10.3342/kjorl-hns.2009.52.11.885

Abstract

BACKGROUND AND OBJECTIVES
The role of Staphylococcus aureus enterotoxin B (SEB) in allergic rhinitis remains controversial. We used a mouse model of airway allergy to determine the effects of nasal instillation of SEB on allergic inflammation. MATERIALS AND METHOD: BALB/c mice were intranasally sensitized with compound of Aspergillus melleus proteinase (AMp) and ovalbumin (ova) in the presence (group 3) or absence of SEB (group 2). Control mice were intranasally sensitized with either phosphate buffered saline (PBS)(group 1) or SEB (group 4) alone. The production of serum antigen-specific antibodies and total IgE, and concentrations of cytokines including interleukin-4 (IL-4), IL-5, interferon-gamma (IFN-gamma) in nasal lavage fluid (NLF) and broncoalveolar lavage fluid (BALF) were compared among the groups. Pathologic studies for eosinophilic infiltrations in the nasal mucosa and peribronchial area were also conducted.
RESULTS
Serum ova-specific immunoglobulin E (IgE) & immunoglobulin G1 (IgG1) were significantly increased in group 2 and 3 compared to group 1. The concentrations of IL-4 in BALF were significantly increased in group 2, 3, and 4 compared to group 1. The concentrations of IL-5 in BALF and NLF were significantly increased in Group 2, 3, and 4 compared to group 1. The concentrations of IFN-gamma were significantly increased in group 3 and 4 compared to group 2 although there was no significant difference among the groups in NLF. Group 2 demonstrates increased infiltration of eosinophils in the nasal mucosa and peribronchial area compared to group 3 and 4.
CONCLUSION
These results suggest that the nasal exposure to SEB did not aggravate inflammation in allergic mice.

Keyword

Allergic rhinitis; Staphylococcus; Enterotoxin; Eosinophil

MeSH Terms

Animals
Antibodies
Aspergillus
Cytokines
Enterotoxins
Eosinophils
Hypersensitivity
Immunoglobulin E
Immunoglobulins
Inflammation
Interferon-gamma
Interleukin-4
Interleukin-5
Mice
Nasal Lavage Fluid
Nasal Mucosa
Ovalbumin
Rhinitis
Rhinitis, Allergic, Perennial
Staphylococcus
Staphylococcus aureus
Therapeutic Irrigation
Antibodies
Cytokines
Enterotoxins
Immunoglobulin E
Immunoglobulins
Interferon-gamma
Interleukin-4
Interleukin-5
Ovalbumin