J Korean Acad Periodontol.  2004 Mar;34(1):205-221. 10.5051/jkape.2004.34.1.205.

A Comparative Study of Gene Expression Patterns of Periodontal Ligament Cells and Gingival Fibroblasts using the cDNA Microarray

• Jeon CY
• Park JW
• Lee JM
• Suh JY

• KMID: 1962491
• DOI: http://doi.org/10.5051/jkape.2004.34.1.205

Abstract

Periodontal ligament(PDL) cells have been known as playing an important roles in periodontal regeneration and gingival fibroblasts are also important to periodontal regeneration by forming connective tissue attachment. There were rare studies about the gene expression patterns of PDL cells and gingival fibroblasts, therefore in this study, we tried cDNA microarray-based gene expression monitoring to explain the functional differences of PDL cells and gingival fibroblasts in vivo and to confirm the characteristics of PDL cells. Total RNA were extracted from PDL cells and gingival fibroblasts of same person and same passages, and mRNA were isolated from the total RNA using Oligotex mRNA midi kit(Qiagen) and then fluorescent cDNA probe were prepared. And microarray hybridization were performed. The gene expression patterns of PDL cells and gingival fibroblasts were quite different. About 400 genes were expressed more highly in the PDL cells than gingival fibroblasts and about 300 genes were more highly expressed in the gingival fibroblasts than PDL cells. Compared growth factor- and growth factor receptor-related gene expression patterns of PDL cells with gingival fibroblasts, IGF-2, IGF-2 associated protein, nerve growth factor, placental bone morphogenic protein, neuron-specific growth- associated protein, FGF receptor, EGF receptor-related gene and PDGF receptor were more highly expressed in the PDL cells than gingival fibroblasts. The results of collagen gene expression patterns showed that collagen type I, type III, type VI and type VII were more highly expressed in the PDL cells than gingival fibroblasts, and in the gingival fibroblasts collagen type V, XII were more highly expressed than PDL cells. The results of osteoblast-related gene expression patterns showed that osteoblast specific cysteine-rich protein were more highly expressed in the PDL cells than gingival fibroblasts. The results of cytoskeletal proteins gene expression patterns showed that alpha-smooth muscle actin, actin binding protein, smooth muscle myosin heavy chain homolog and myosin light chain were more highly expressed in the PDL cells than gingival fibrobalsts, and beta-actin, actin-capping protein(beta subunit), actin- related protein Arp3(ARP) and myosin class I(myh-1c) were more highly expressed in the gingival fibroblasts than PDL cells. Osteoprotegerin/osteoclastogenesis inhibitory factor(OPG/OCIF) was more highly expressed in the PDL cells than gingival fibroblasts. According to the results of this study, PDL cells and gingival fibroblasts were quite different gene expression patterns though they are the fibroblast which have similar shape. Therefore PDL cells & gingival fibroblasts are heterogeneous populations which represent distinct characteristics. If more studies about genes that were differently expressed in each PDL cells & gingival fibroblasts would be performed in the future, it would be expected that the characteristics of PDL cells would be more clear.

Keyword

cDNA microarray; PDL Cells; HGF; Characteristics; genes

MeSH Terms

Actins
Carrier Proteins
Collagen
Collagen Type I
Collagen Type V
Connective Tissue
Cytoskeletal Proteins
DNA, Complementary*
Epidermal Growth Factor
Fibroblasts*
Gene Expression Profiling
Gene Expression*
Humans
Insulin-Like Growth Factor II
Muscle, Smooth
Myosin Heavy Chains
Myosin Light Chains
Myosins
Nerve Growth Factor
Oligonucleotide Array Sequence Analysis*
Osteoblasts
Periodontal Ligament*
Receptors, Fibroblast Growth Factor
Receptors, Platelet-Derived Growth Factor
Regeneration
RNA
RNA, Messenger
Actins
Carrier Proteins
Collagen
Collagen Type I
Collagen Type V
Cytoskeletal Proteins
DNA, Complementary
Epidermal Growth Factor
Insulin-Like Growth Factor II
Myosin Heavy Chains
Myosin Light Chains
Myosins
Nerve Growth Factor
RNA
RNA, Messenger
Receptors, Fibroblast Growth Factor
Receptors, Platelet-Derived Growth Factor