J Korean Acad Periodontol.  1997 Jun;27(2):291-303. 10.5051/jkape.1997.27.2.291.

Biological Characteristics of Human Periodontal Ligament Cells

• Park GW
• Shin HS
• You HK

• KMID: 1959402
• DOI: http://doi.org/10.5051/jkape.1997.27.2.291

Abstract

Periodontal ligament cells may have a role in the regulation of hard and soft periodontal tissues, but their specific function has not yet to be determined. To evaluate further their role in periodontal regeneration, they were examined for osteoblast-like behavior. Periodontal ligament cells and gingival fibroblasts were primarily cultured from extracted premolar with non-periodontal diseases. Cells were cultured with DMEM at 37degrees C, 5% CO2, 100% humidity incubator, and as a measure of cell characterization, it was examined that the morphology, alkaline phosphatase activity, collagen synthesis, and immunocytochemistry for osteonectin, osteocalcin, and collagen type I. Healthy periodontal ligament cells has more osteoblastic-like cell property in alkaline phosphatase activity, and collagen synthesis than gingival fibroblast. Immunocytochemistry localization explained that calcitonin were expressed in periodontal ligament cells only, and osteonectin and type I collagen were produced in both cells simultaneously. This results indicate that the growth characteristics of periodontal ligament cells and gingival fibroblasts exhibit some differences in proliferative rates and biochemical synthesis. The differences may help to calrify the role such cells play in the regenearation of periodontal tissues.

MeSH Terms

Alkaline Phosphatase
Bicuspid
Calcitonin
Collagen
Collagen Type I
Fibroblasts
Humans*
Humidity
Immunohistochemistry
Incubators
Osteocalcin
Osteonectin
Periodontal Ligament*
Population Characteristics*
Regeneration
Alkaline Phosphatase
Calcitonin
Collagen
Collagen Type I
Osteocalcin
Osteonectin