Korean J Physiol Pharmacol.  2000 Oct;4(5):339-346.

p38 MAPK and NF-kappaB are required for LPS-induced RANTES production in immortalized murine microglia (BV-2)

• Jang SB
• Lee KH

Abstract

Using murine immortalized microglial cells (BV-2), we examined the regulation of RANTES production stimulated by lipopolysaccharide (LPS), focusing on the role of mitogen-activated protein kinase (MAPK) and nuclear factor (NF)-kappaB. The result showed that RANTES (regulated upon activation of normal T cell expressed and secreted) was induced at the mRNA and protein levels in a dose- and time-dependent manner in response to LPS. From investigations of second messenger pathways involved in regulating the secretion of RANTES, we found that LPS induced phosphorylation of extracellular signal-regulated kinase (Erk), p38 MAPK and c-Jun-N-terminal kinase (JNK), and activated NF-kappaB. To determine whether this MAPK phosphorylation is involved in LPS-stimulated RANTES production, we used specific inhibitors for p38 MAPK and Erk, SB 203580 and PD 98059, respectively. LPS-induced RANTES production was reduced approximately 80% at 25 micrometer of SB 203580 treatment. But PD 98059 did not affect RANTES production. Pyrrolidine-dithiocarbamate (PDTC), NF-kappaB inhibitor, reduced RANTES secretion. These results suggest that LPS-induced RANTES production in microglial cells (BV-2) is mainly mediated by the coordination of p38 MAPK and NF-kappaB cascade.

Keyword

Microglia; BV-2; RANTES; LPS; MAPK; NF-kB

MeSH Terms

Chemokine CCL5*
Microglia*
NF-kappa B*
p38 Mitogen-Activated Protein Kinases*
Phosphorylation
Phosphotransferases
Protein Kinases
RNA, Messenger
Second Messenger Systems
Chemokine CCL5
NF-kappa B
Phosphotransferases
Protein Kinases
RNA, Messenger
p38 Mitogen-Activated Protein Kinases