Lab Anim Res.  2011 Dec;27(4):317-325. 10.5625/lar.2011.27.4.317.

Inhibitory effect of Suaeda asparagoides (Miq.) extract on the motility of rat gastric antrum is mediated by beta-adrenoceptor

Affiliations
  • 1College of Veterinary Medicine, Kyungpook National University, Daegu, Korea. twkim@mail.knu.ac.kr
  • 2College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu, Korea.
  • 3Reclaimed Land Agriculture Research Division, National Institute of Crop Science, RDA, Iksan, Korea.
  • 4Department of Internal Medicine, Catholic University of Daegu School of Medicine, Daegu, Korea.
  • 5Department of Internal Medicine, Keimyung University School of Medicine, Daegu, Korea.
  • 6Department of Physiology and Neuroscience, College of Dentistry, Gangneung-Wonju National University, Gangneung, Korea.

Abstract

Suaeda asparagoides (Miq.) has long been used as a Korean folk herbal medicine for the treatment of functional gastrointestinal disorders. However, reports on its pharmacological activity on gastrointestinal motility are scarce. The present study investigated the effects of Suaeda asparagoides water fraction of the extract (SAWF) on antral motility in vitro. Muscle strips from rat gastric antrum were set up in an organ bath in a circular orientation. SAWF (100 microg/mL) inhibited the spontaneous contraction of antral circular muscle strips. These inhibitory effects were not significantly affected by tetrodotoxin (1 microM), N omega-Nitro-L-arginine methyl ester hydrochloride (100 microM), 1H-(1,2,4)oxadiazolo(4,3-a)quinoxalin-1-one (10 microM), ryanodine (10 microM) and phentolamine (10 microM). SAWF-induced inhibition was mostly restored by cyclopiazonic acid (10 microM). Furthermore, the beta-adrenergic receptor antagonist, propranolol (10 microM), abolished SAWF-induced inhibition. These results suggest that SAWF may exert its activity on gastrointestinal smooth muscle via a-adrenergic receptors and sarcoplasmic reticulum Ca2+ ATPase.

Keyword

Suaeda asparagoides; gastric antrum; spontaneous contraction; beta-adrenoceptor; SERCA

MeSH Terms

Animals
Baths
Calcium-Transporting ATPases
Carbamates
Chenopodiaceae
Contracts
Gastrointestinal Diseases
Gastrointestinal Motility
Herbal Medicine
Indoles
Muscle, Smooth
Muscles
Organometallic Compounds
Orientation
Oxadiazoles
Phentolamine
Propranolol
Pyloric Antrum
Quinoxalines
Rats
Ryanodine
Sarcoplasmic Reticulum
Tetrodotoxin
Water
Calcium-Transporting ATPases
Carbamates
Indoles
Organometallic Compounds
Oxadiazoles
Phentolamine
Propranolol
Quinoxalines
Ryanodine
Tetrodotoxin
Water

Figure

  • Figure 1 Biphasic effects of SAWF on the spontaneous contractility of rat gastric antral circular muscle. Low concentrations (1-10 mg/mL) of SAWF increased the motility of gastric antral circular muscle, whereas high concentrations (>10 mg/mL) repressed motility.

  • Figure 2 Effect of Na+ channel blocker, tetrodotoxin (TTX), on SAWF-induced responses of strips from circular muscles of rat gastric antrum. (A and B) Antral smooth muscle strip responses induced by SAWF (100 µg/mL) alone (A) or in the presence of TTX (1 µM) (B). (C) This experiment performed in same strip. Actual values for amplitude inhibition effect of SAWF alone and TTX pretreated antral muscle strips. (D) Pretreatment of TTX did not significant affect on the amplitude ratio of the remnant phasic contraction after SAWF administration. Values are mean±SEM. *Significantly different from previous column, P<0.05 (n=6).

  • Figure 3 Effect of L-NAME on SAWF evokes relaxation of rat gastric antrum. (A) SAWF-induced inhibition on antral spontaneous phasic contraction. (B) Panel for the effect of SAWF on L-NAME pretreated antral muscle strip. (C) Summary for the actual values of control, SAWF and L-NAME treatment groups. (D) Pretreatment of L-NAME (100 µM) showed a slight tendency to increase the amplitude ratio of the remnant phasic contraction after SAWF administration without significance. Values are mean±SEM. *Significantly different from previous column, P<0.05 (n=6).

  • Figure 4 Effect of ODQ on SAWF induced inhibition on amplitude of rat gastric antral muscle phasic contraction. (A) Panel for SAWF-induced inhibition on the amplitude of rat antral spontaneous phasic contraction (B) Panel for the effect of SAWF on ODQ pretreated antral muscle strip. (C) Summary for the actual value of control, SAWF, and ODQ treatment groups. (D) ODQ (10 µM) slightly but significantly increased the amplitude ratio of the remnant spontaneous contraction after SAWF administration. Values are mean±SEM. *Significantly different from previous column, P<0.05 (n=6).

  • Figure 5 Effect of ryanodine on SAWF induced inhibition on amplitude of rat gastric antral muscle phasic contraction. (A) Panel for SAWF induced inhibition on the amplitude of spontaneous phasic contraction (B) Panel for the effect of SAWF on ryanodine pretreated antral muscle strip. The bars over the panels indicate the duration of administration of the extract ryanodine. (C) Summary for the control, SAWF and ryanidine treatment groups. (D) Ryanodine (10 µM) did not affect SAWF induced inhibition ratio on the amplitude of rat antral muscle phasic contraction. Values are mean±SEM. *Significantly different from previous column, P<0.05 (n=6).

  • Figure 6 Effects of CPA on SAWF evoked inhibition of antral muscle phasic contraction. (A) Panel for SAWF-induced inhibition on the amplitude of spontaneous phasic contraction. (B) Sarcoplasmic reticulum Ca2+-ATPase inhibitor, cyclopiazonic acid (CPA, 10 µM), did not affect on the gastric antral motiltity. (C) Actual values on SAWF alone & in the presence of CPA with their respective controls. (D) CPA mostly protected the amplitude of the phasic contraction from SAWF inhibition. Values are mean±SEM. *Significantly different from previous column, P<0.05 (n=6).

  • Figure 7 Effects of α- and β-adrenergic receptor antagonists on SAWF induced inhibition. (A) Phentolamine, α-adrenergic receptor (10 µM) did not affect SAWF evoked inhibition of rat antral muscle phasic contraction. (B) However, propranolol, a β-adrenergic receptor antagonist (10 µM), completely abolished SAWF induced inhibition of rat antral muscle phasic contraction (B) Values are mean±SEM. *Significantly different from previous column, P<0.05 (n=6).


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