Lab Anim Res.  2011 Dec;27(4):283-291. 10.5625/lar.2011.27.4.283.

Basic data on the hematology, serum biochemistry, urology, and organ weights of beagle dogs

Affiliations
  • 1Veterinary Medical Center, Chungbuk National University, Cheongju, Korea. kang6467@cbu.ac.kr
  • 2Biotoxtech Co., Ltd., Ochang, Korea.
  • 3Department of Biomaterials Science, College of Natural Resources & Life Science, Pusan National University, Miryang, Korea.

Abstract

This study was conducted to provide basic data on physiological and hematological characteristics, and organ weights of beagle dogs. A total of 237 beagle dogs were used to determine differences in physiological and hematological parameters, and organ weights depending on sex and age. The respiratory rate of both sexes tended to increase as they grew older and the female heart rate was slightly higher than that of males. Male and female body weights increased rapidly to 33 weeks old followed by a gradual increase to 41-weeks-old. The relative weight of the brain was negatively correlated with body weight, whereas the weight of reproductive organs was positively correlated with body weight. The platelet count of female dogs was slightly higher than that of males. The red blood cell, hemoglobin, and hematocrit of both sexes increased non-significantly with age. In the leukocyte differential count, the neutrophils, and eosinophils of both sexes tended to increase as they grew older, whereas basophils, lymphocytes, and monocytes decreased. In the serum biochemical profiles, alkaline phosphatase was slightly higher in males than females, while the total cholesterol of female dogs at 9-months-old was higher than that of males at the same age. Other biochemical components, including alanine aminotransferase, blood urea nitrogen, creatinine, triglyceride, and total protein increased non-significantly with age in both sexes. To conclude, we observe no significant physiological or hematological differences with sex or age, although decreasing and increasing trends were detected with some parameters. These data provide valuable reference indices of the normal physiological and hematological characteristics of beagle dogs, which should prove useful in toxicological and pharmacological studies.

Keyword

Physiology; hematology; serum biochemistry; age; organ weight; beagle dog

MeSH Terms

Alanine Transaminase
Alkaline Phosphatase
Animals
Basophils
Biochemistry
Blood Urea Nitrogen
Body Weight
Brain
Cholesterol
Creatinine
Dogs
Eosinophils
Erythrocytes
Female
Heart Rate
Hematocrit
Hematology
Hemoglobins
Humans
Leukocytes
Lymphocytes
Male
Monocytes
Neutrophils
Organ Size
Platelet Count
Respiratory Rate
Urology
Alanine Transaminase
Alkaline Phosphatase
Cholesterol
Creatinine
Hemoglobins

Figure

  • Figure 1 Changes in body weights of male and female dogs.


Reference

1. Steinmetz KL, Spack EG. The basics of preclinical drug development for neurodegenerative disease indications. BMC Neurol. 2009; 9(Suppl 1):S2. PMID: 19534731.
Article
2. Smith D, Broadhead C, Descsotes G, Fosse R, Hack R, Krauser K, Pfister R, Phillips B, Rabemampianina Y, Sanders J, Sparrow S, Stephan-Gueldner M, Jacobsen SD. Preclinical safety evaluation using nonrodent species: an industry/welfare project to minimize dog use. ILAR J. 2002; 43(Suppl):S39–S42. PMID: 12388850.
Article
3. Meunier LD. Selection, acclimation, training, and preparation of dogs for the research setting. ILAR J. 2006; 47(4):326–347. PMID: 16963813.
Article
4. Smith D, Combes R, Depelchin O, Jacobsen SD, Hack R, Luft J, Lammens L, von Landenberg F, Phillips B, Pfister R, Rabemampianina Y, Sparrow S, Stark C, Stephan-Gueldner M. Optimising the design of preliminary toxicity studies for pharmaceutical safety testing in the dog. Regul Toxicol Pharmacol. 2005; 41(2):95–101. PMID: 15698532.
Article
5. Phillips B, Smith D, Combes R, Descotes G, Dyring Jacobsen S, Hack R, Kemkowski J, Krauser K, PWster R, Rabemampianina Y, Sparrow S, Stephan-Gueldner M, von Landenberg F. An approach to minimise dog use in regulatory toxicology: production of a best practice guide to study design. Altern Lab Anim. 2004; 32(S1):447–451.
Article
6. Swallow J, Anderson D, Buckwell AC, Harris T, Hawkins P, Kirkwood J, Lomas M, Meacham S, Peter A, Prescott M, Owen S, Quest R, Sutcliffe R, Thompson K. Guidance on the transport of laboratory animals: Report of the transport working group established by the Laboratory Animal Science Association (LASA). Lab Anim. 2005; 39:1–39. PMID: 15703122.
7. Kuhn G, Lichtwald K, Hardegg W, Abel HH. The effect of transportation stress on circulating corticosteroids, enzyme activities and hematological values in laboratory dogs. J Exp Anim Sci. 1991; 34:99–104. PMID: 1742341.
8. Burkholder WJ. Use of body condition scores in clinical assessment of the provision of optimal nutrition. J Am Vet Med Assoc. 2000; 217:650–654. PMID: 10976293.
Article
9. Fillman-Holliday D, Landi MS. Animal care best practices for regulatory testing. ILAR J. 2002; 43:S49–S58. PMID: 12388852.
Article
10. Ha CS, Son HY, Kang BY, Cha SW, Park JI, Han SS. A study of hematology and serum chemistry in beagle dogs. Korean J Lab Anim Sci. 1995; 11(2):153–158.
11. Balls M, Amcoff P, Bremer S, Casati S, Coecke S, Clothier R, Combes R, Corvi R, Curren R, Eskes C, Fentem J, Gribaldo L, Halder M, Hartung T, Hoffmann S, Schectman L, Scott L, Spielmann H, Stokes W, Tice R, Wagner D, Zuang V. The principles of weight of evidence validation of test methods and testing strategies. Altern Lab Anim. 2006; 34(6):603–620. PMID: 17266393.
Article
12. Jeong EJ, Han SC, Cha SW, Lee HS, Ha CS, Kim CY. Hematological and blood biochemical values of laboratory beagle dogs. Lab Anim Res. 2007; 23(3):223–229.
13. Matsuzawa T, Nomura M, Unno T. Clinical pathology reference ranges of laboratory animals. Working Group II, Nonclinical Safety Evaluation Subcommittee of the Japan Pharmaceutical Manufacturers Association. J Vet Med Sci. 1993; 55(3):351–362. PMID: 8357905.
14. Ikeuchi J, Yoshizaki T, Hirata M. Plasma biochemistry values of young beagle dogs. J Toxicol Sci. 1991; 16:49–59. PMID: 1886172.
Article
15. Matsuzawa T, Nomura M, Yonezawa H, Unno T. Selection of appropriate parameters, use of a quality control concept, and suitable statistical analyses for clinical pathology examination of animals in toxicity studies: results of a current survey by the Japanese Pharmaceutical Manufacturers Association. Comp Haematol Int. 1995; 5:196–200.
Article
16. Gonder JC, Laber K. A renewed look at laboratory rodent housing and management. ILAR J. 2007; 48(1):29–36. PMID: 17170493.
Article
17. Sharp J, Zammit T, Azar T, Lawson D. Stress-like responses to common procedures in individually and grouphoused female rats. Contemp Top Lab Anim Sci. 2003; 42(1):9–18. PMID: 12580569.
18. Ferasin L, Ferasin H, Little CJL. Lack of correlation between canine heart rate and body size in veterinary clinical practice. J Small Anim Pract. 2010; 51:412–418. PMID: 20553373.
Article
19. Nunamaker E, Newhall K, Thompson C, Lucas A, Owens J, Sherman JG. Safety evaluation and treatment affect of LY2190416, a CB-1 antagonist/inverse agonist in growing beagle dogs. J Vet Pharmacol Ther. 2011; doi: 10.1111/j.1365-2885.2011.01280.x.
Article
20. Shin JW, Park HJ, Kwon M, Son CG. Scientific evaluation of the herbal medicine CGX in beagle dogs. Food Chem Toxicol. 2010; 48:743–749. PMID: 20034534.
21. Wolford ST, Schroer RA, Gohs FX, Gallo PP, Brodeck M, Falk HB, Ruhren R. Reference range data base for serum chemistry and hematology values in laboratory animals. J Toxicol Environ Health. 1986; 18:161–188. PMID: 3712484.
Article
22. Posen S, Doherty E. The measurement of serum alkaline phosphatase in clinical medicine. Adv Clin Chem. 1981; 22:163–245. PMID: 7027760.
Article
23. Amacher DE, Higgins CV, Schomaker SJ, Clay RJ. Further characterization of serum alkaline phosphatase from male and female beagle dogs. Enzyme. 1989; 42:1–7. PMID: 2776712.
24. Uchiyama T, Tokoi K, Deki T. Successive changes in the blood composition of experimental normal beagle dogs associated with age. Jikken Dobutsu. 1985; 34(4):367–377. PMID: 4085564.
25. Bayard SP. Another look at the statistical analysis of changes during storage of serum specimens. Health Lab Sci. 1974; 11:45–49. PMID: 4808617.
26. Caisey JD, King DJ. Clinical chemical values for some common laboratory animals. Clin Chem. 1980; 26:1877–1879. PMID: 7438435.
Article
27. Kaspar LV, Norris WP. Serum chemistry values of normal dogs (beagles): associations with age, sex, and family line. Lab Anim Sci. 1977; 27(6):980–985. PMID: 599888.
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