J Cancer Prev.  2019 Jun;24(2):123-128. 10.15430/JCP.2019.24.2.123.

Esculetin Prevents the Induction of Matrix Metalloproteinase-1 by Hydrogen Peroxide in Skin Keratinocytes

• Zhen AX
• Piao MJ
• Kang KA
• Fernando P
• Kang HK
• Koh YS
• Hyun JW

• KMID: 2454671
• DOI: http://doi.org/10.15430/JCP.2019.24.2.123

Abstract

BACKGROUND
Reactive oxygen species (ROS) are involved in various cellular diseases. Excessive ROS can cause intracellular oxidative stress, resulting in a calcium imbalance and even aging. In this study, we evaluated the protective effect of esculetin on oxidative stress-induced aging in human HaCaT keratinocytes.
METHODS
Human keratinocytes were pretreated with esculetin for 30 minutes and treated with Hâ‚‚Oâ‚‚. Then, the protective effects on oxidative stress-induced matrix metalloproteinase (MMP)-1 were detected by Flou-4-AM staining, reverse transcription-PCR, Western blotting, and quantitative fluorescence assay.
RESULTS
Esculetin prevented Hâ‚‚Oâ‚‚-induced aging by inhibiting MMP-1 mRNA, protein, and activity levels. In addition, esculetin decreased abnormal levels of phospho-MEK1, phospho-ERK1/2, phospho-SEK1, phospho-JNK1/2, c-Fos, and phospho-c-Jun and inhibited activator protein 1 binding activity.
CONCLUSIONS
Esculetin prevented excessive levels of intracellular calcium and reduced the expression levels of aging-related proteins.

Keyword

Esculetin; Reactive oxygen species; Matrix metalloproteinase-1; Aging

MeSH Terms

Aging
Blotting, Western
Calcium
Fluorescence
Humans
Hydrogen Peroxide*
Hydrogen*
Keratinocytes*
Matrix Metalloproteinase 1*
Oxidative Stress
Reactive Oxygen Species
RNA, Messenger
Skin*
Transcription Factor AP-1
Calcium
Hydrogen
Hydrogen Peroxide
Matrix Metalloproteinase 1
RNA, Messenger
Reactive Oxygen Species
Transcription Factor AP-1