Korean J Dermatol.  1999 Mar;37(3):352-357.

The Effects of Hydrogen Peroxide on the Migration and Proliferation of the Human Keratinocytes during Wound Healing

• Park HJ
• Lee JH

Abstract

BACKGROUND: Various topical antisepties are often used for postoperative care of surgical wounds. But the effect of these agent on wound healing remains an area of debate.
OBJECTIVE
The purpose of this study was to evaluate the biological effects of hydrogen peroxide on human keratinocytes in vitro, specifically on keratinocyte viability, migration and proliferation.
METHODS
To evaluate the effects of hydrogen peroxide on keratinocyte viability, migration and proliferation, sulforhodamine B test, migration assay, and thymidine incorporation assay were performed, respectively. Diluted solutions of 30% hydrogen peroxide( X 50, X 100, X 500, X 1000) were used as testing material and growth medium alone as a control.

Keyword

Hydrogen peroxide; Wound healing; keratinocyte migration & proliferation

MeSH Terms

Humans*
Hydrogen Peroxide*
Hydrogen*
Keratinocytes*
Postoperative Care
Thymidine
Wound Healing*
Wounds and Injuries*
Hydrogen
Hydrogen Peroxide
Thymidine