J Cancer Prev.  2017 Dec;22(4):228-233. 10.15430/JCP.2017.22.4.228.

Protective Effect of Allium tuberosum Extract on Vascular Inflammation in Tumor Necrosis Factor-α-induced Human Vascular Endothelial Cells

• Hur HJ
• Lee AS

• KMID: 2434284
• DOI: http://doi.org/10.15430/JCP.2017.22.4.228

Abstract

BACKGROUND
Endothelial adhesion molecule expression induced by pro-inflammatory cytokine plays an important role in vascular endothelial cell injury, leading to vascular disease. Allium tuberosum (AT), which is used as a functional food, has a thrombolytic effect. It contains vitamin A, vitamin C, carbohydrate, calcium, iron, and phosphorus. There are many carotenes that turn into vitamin A in the body. Also, it helps blood circulation and stimulates metabolism. The purpose of the this study was to estimate the anti-inflammatory effects of the AT extract.
METHODS
Human vascular endothelial cells were pre-treated with 100 μg/mL AT extract for 30 minutes and subsequently co-treated with TNF-α (10 ng/mL) and AT extract (100 μg/mL) for 1, 4, and 6 hours. After treatment, the cells were lysed and used for quantitative reverse transcription PCR, Western blot analysis, and monocyte adhesion assay.
RESULTS
We examined the effect of the AT extract on inflammatory gene expression in TNF-α-induced human umbilical vein endothelial cells (HUVECs). The extract reduced the expression levels of mRNA and protein of intercellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule (VCAM)-1 in TNF-α-stimulated HUVECs. It also inhibited the TNF-α-induced phosphorylation of the NF-κB p65 subunit and degradation of IκBα. Furthermore, the AT extract prevented the increased adhesion capacity of monocyte to TNF-α-stimulated vascular endothelial cells by reducing ICAM-1 and VCAM-1 expression.
CONCLUSIONS
The AT extract has preventive and anti-inflammatory effect against vascular disease and has potential for supporting prevention against the early process of atherosclerosis.

Keyword

Allium tuberosum extract; Vascular cell adhesion molecule-1; Intercellular adhesion molecule-1; Inflammation; NF-kappa B

MeSH Terms

Allium*
Ascorbic Acid
Atherosclerosis
Blood Circulation
Blotting, Western
Calcium
Carotenoids
Chive*
Endothelial Cells*
Functional Food
Gene Expression
Human Umbilical Vein Endothelial Cells
Humans*
Inflammation*
Intercellular Adhesion Molecule-1
Iron
Metabolism
Monocytes
Necrosis*
NF-kappa B
Phosphorus
Phosphorylation
Polymerase Chain Reaction
Reverse Transcription
RNA, Messenger
Vascular Cell Adhesion Molecule-1
Vascular Diseases
Vitamin A
Ascorbic Acid
Calcium
Carotenoids
Intercellular Adhesion Molecule-1
Iron
NF-kappa B
Phosphorus
RNA, Messenger
Vascular Cell Adhesion Molecule-1
Vitamin A