Korean J Nephrol.
1998 May;17(3):383-390.
The Effect of Erythropoietin on the Production of Endothelin in Human Glomerular and Umbilical Endothelial Cells
- Affiliations
-
- 1College of Medicine, University of Ulsan Department of Internal Medicine, Seoul, Korea.
- 2College of Medicine, University of Ulsan Department of Urology, Seoul, Korea.
- 3College of Medicine, University of Ulsan Department of Biochemistry, Seoul, Korea.
Abstract
- The administration of erythropoietin for renal failure patients is frequently associated with a mild-to-marked rise in arterial blood pressure. The erythropoietin-induced hypertension has been variably attributed to the rise in erythrocyte concentration and/or a direct or indirect pressor action of erythropoietin on vascular smooth muscle. Especially erythropoietin-induced hypertension may be mediated by increased production of the potent vasoactive peptide endothelin. This study was designed to determine the effect of erythropoietin to the production of endothelin in human glomerular endothelial cells and human umbilical vein endothelial cells. ELISA method was used to measure the endothelin- 1, after 4 X 10(4) endothelial cells were grown to confluency on 24-well plates, in which erythropoietin 25u/ml was incubated for 24 hours. The results showed that the endothelin production in human glomerular endothelial cells was insignificant (116.0+/-14.0%, P>0.05, mean+/-S.E., n=5, each n means the mean of duplicate experiments), but the endothelin production in human umbilical endothelial cells was increased after 25u/ml erythropoietin treatment(126.5+/-15.2%, P<0.05, mean+/-S.E., n=4). In glomerular endothelial cells, TGF-beta(0.5ng/ml) increased the production of endothelin(218.8+/-57.2%, P<0.01, mean+/-S.E., n=5), also it looks like that TNF-alpha and thrombin might increase the production of endothelin according to the concentration. In conclusion, the responsiveness of endothelial cells to erythropoietin may be different according to the cell type, and glomerular endothelial cells could increase the production of endothelin, if appropriate stimuli were given.
Keyword
MeSH Terms
-
Arterial Pressure
Endothelial Cells*
Endothelins*
Enzyme-Linked Immunosorbent Assay
Erythrocytes
Erythropoietin*
Human Umbilical Vein Endothelial Cells
Humans*
Hypertension
Muscle, Smooth, Vascular
Renal Insufficiency
Thrombin
Transforming Growth Factor beta
Tumor Necrosis Factor-alpha
Endothelins
Erythropoietin
Thrombin
Transforming Growth Factor beta
Tumor Necrosis Factor-alpha
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- The effect of the sera from severe preeclamptic patients on endothelial cell viability and endothelin-1 in cultured human umbilical vein endothelial cells
- High glucose stimulates the expression of erythropoietin in rat glomerular epithelial cells
- Quantification of endothelin-1 in human umbilical venous endothelial cell culture supernatants of small for gestational age and preeclampsia neonates
- The Effect of VEGF (Vascular endothelial growth factor) on Endothelial Cells in Pathogenesis of Preeclampsia
- Culture of human endothelial cells derived from umbilical veins
