Korean J Pediatr.  2007 Dec;50(12):1194-1199. 10.3345/kjp.2007.50.12.1194.

Quantification of endothelin-1 in human umbilical venous endothelial cell culture supernatants of small for gestational age and preeclampsia neonates

• Cho WK
• Kim SY
• Chun CS

• KMID: 2279450
• DOI: http://doi.org/10.3345/kjp.2007.50.12.1194

Abstract

PURPOSE: It was generally accepted now a days that the pathogenesis of preeclampsia, small for gestational age (SGA), intrauterine growth retardation and fetal origin of adult diseases were related with a endothelial cell dysfunction. The purpose of this study was to know the relation of such diseases by assessing the level of endothelin-1.
METHODS
SGA babies, newborns of preeclampsia and normal control mother were included in this study. Isolated endothelial cells were centrifugated and mixed with media in 37, 5% CO2 to obtain confluent monolayer of cultured human umbilical venous endothelial cell (HUVEC). Endothelin-1 levels were determined by Endothelin-1 colorimetric (EIA) Kits. We examined the endothelin-1 level in the HUVEC supernatants from SGA baby, and newborns from preeclampsia as well as normal mother. Also, we compared the endothelin-1 level of cultured normal HUVEC incubated with serum from cord blood of SGA, babies of preeclampsia or normal control mother.
RESULTS
The endothelin-1 levels in cultured HUVEC supernatants of three groups showed no significant difference but the endothelin-1 levels of cultured normal HUVEC incubated with serum from preeclampsia mother or SGA mother was significantly higher than those from newborns of control mothers (P<0.05).
CONCLUSION
These findings suggest that there may be the factor which affect the endothelin-1 level in serum of cord blood from SGA and preeclampsia.

Keyword

Preeclampsia; Small for gestational age; Endothelin-1; Umbilical vein; Endothelial cell

MeSH Terms

Adult
Endothelial Cells*
Endothelin-1*
Fetal Blood
Fetal Growth Retardation
Gestational Age*
Humans*
Infant, Newborn*
Mothers
Pre-Eclampsia*
Umbilical Veins
Endothelin-1