Int Neurourol J.
2018 Oct;22(Suppl 3):S147-S155. 10.5213/inj.1836226.113.
Treadmill Exercise Improves Motor Function by Suppressing Purkinje Cell Loss in Parkinson Disease Rats
- Affiliations
-
- 1Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea.
- 2Department of Anesthesiology and Pain Medicine, Kyung Hee Medical Center, College of Medicine, Kyung Hee University, Seoul, Korea.
- 3School of Global Sport Studies, Korea University, Sejong, Korea.
- 4Division of Leisure & Sports Science, Department of Exercise Prescription, Dongseo University, Busan, Korea.
- 5Department of Sport & Health Science, College of Natural Science, Sangmyung University, Seoul, Korea.
- 6Department of Urology, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Korea.
- 7Department of Urology, Gil Medical Center, Gachon University School of Medicine, Incheon, Korea. kimcho99@gilhospital.com
- KMID: 2426693
- DOI: http://doi.org/10.5213/inj.1836226.113
Abstract
- PURPOSE
Rotenone is the most widely used neurotoxin for the making Parkinson disease (PD) animal model. The neurodegenerative disorder PD shows symptoms, such as slowness of movements, tremor at resting, rigidity, disturbance of gait, and instability of posture. We investigated whether treadmill running improves motor ability using rotenone-caused PD rats. The effect of treadmill running on PD was also assessed in relation with apoptosis of cerebellar Purkinje cells.
METHODS
Treadmill running was applied to the rats in the exercise groups for 30 minutes once a day for 4 weeks, starting 4 weeks after birth. We used rota-rod test for the determination of motor coordination and balance. In this experiment, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining, immunohistochemistry for calbindin, glial fibrillary acidic protein (GFAP), Iba-1, and western blot analysis for Bax and Bcl-2 were performed.
RESULTS
Treadmill running enhanced motor balance and coordination by preventing the loss of Purkinje cells in the cerebellar vermis. Treadmill running suppressed PD-induced expression of GFAP-positive reactive astrocytes and Iba-1-positive microglia, showing that treadmill running suppressed reactive astrogliosis and microglia activation. Treadmill running suppressed TUNEL-positive cell number and Bax expression and enhanced Bcl-2 expression, demonstrating that treadmill running inhibited the progress of apoptosis in the cerebellum of rotenone-induced PD rats.
CONCLUSIONS
Treadmill running improved motor ability of the rotenone-induced PD rats by inhibiting apoptosis in the cerebellum. Apoptosis suppressing effect of treadmill running on rotenone-induced PD was achieved via suppression of reactive astrocyte and inhibition of microglial activation.
MeSH Terms
-
Animals
Apoptosis
Astrocytes
Blotting, Western
Calbindins
Cell Count
Cerebellar Vermis
Cerebellum
Gait
Glial Fibrillary Acidic Protein
Immunohistochemistry
Microglia
Models, Animal
Neurodegenerative Diseases
Parkinson Disease*
Parturition
Posture
Purkinje Cells*
Rats*
Rotenone
Running
Tremor
Glial Fibrillary Acidic Protein
Rotenone
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